Date published: 2025-9-9

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Anticorps NFATc4 (F-3): sc-271091

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Fiches techniques
  • L'Anticorps NFATc4 (F-3) est un monoclonal de souris IgG2b κ, cité dans 1 publications, fourni en 200 µg/ml
  • dirigé contre les acides aminés 125-198 de NFATc4 d'origine human
  • recommandé pour la détection de NFATc4 d'origine human par WB, IP, IF et ELISA
  • Réactifs TransCruz pour une application en ChIP (sc-271091 X, 200 µg/0.1 ml)
  • Voir NFATc4 (B-2): sc-271597 pour d'autres anticorps conjugués NFATc4, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
  • 2b BP-HRP">m-IgG2b BP-HRP est le réactif de détection secondaire préféré pour l'anticorps NFATc4 (F-3) pour les applications WB. Ce réactif est désormais proposé en association avec l'anticorps NFATc4 (F-3)(voir les informations relatives à la commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

NFATc4 (nuclear factor of activated T cells, cytoplasmic, calcineurin-dependent 4) is a member of the nuclear factors of activated T cells DNA-binding transcription complex that influences cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. This complex consists of at least two components: a cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate in the formation of this complex. NFATc4 plays a role in the inducible expression of cytokine genes in T cells, including the induction of IL-2 and IL-4. p38 MAP kinase phosphorylates multiple residues in the NFAT homology domain of NFATc4.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps NFATc4 (F-3)

sc-271091
200 µg/ml
$316.00

NFATc4 (F-3): m-IgG2b BP-HRP Kit

sc-548535
200 µg Ab; 10 µg BP
$354.00

Anticorps NFATc4 (F-3) X

sc-271091 X
200 µg/0.1 ml
$316.00

Is this antibody suited to detect NFAT in cytoplasm and in the nuclei of cells?

Posée par: Sebastian M
Thank you for your question. NFATc4 Antibody (F-3): sc-271091 is suitable for the detection of intracellular NFATc4. Depending on the sample, antigen demasking may be necessary. You can find our recommended protocol for immunofluorescence, IHC and different antigen demasking methods by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunofluorescence-cell-staining
Répondue par: SCBT Heidelberg Support
Date de publication: 2018-12-22

What is the recommended fixation method for immunofluorescence staining with NFATc4 (F-3): sc-271091 monoclonal antibody?

Posée par: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-02-27
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Rated 5 de 5 de par Observed robust band in NFATc4 transfectedObserved robust band in NFATc4 transfected 293T whole cell lysate. -SCBT QC
Date de publication: 2015-02-28
Rated 5 de 5 de par Excellent immunofluorescence cytoplasmicExcellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date de publication: 2015-01-02
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Anticorps NFATc4 (F-3) est évalué 5.0 de 5 de 2.
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