Anticorps NEDD1 (39-J) recommandé pour la détection de NEDD1 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps NEDD1 (39-J): sc-100961

Anticorps NEDD1 (39-J) est évalué 4.2 de 5 de 5.
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5
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Fiches techniques

    • L'Anticorps NEDD1 (39-J) est un monoclonal de souris IgG2b κ, cité dans 5 publications, fourni en 100 µg/ml
    • dirigé contre la NEDD1 recombinante d'origine human
    • recommandé pour la détection de NEDD1 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Can sc-100961: NEDD1 (39-J) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Posée par: Cweed
Thank you for your question. Yes, sc-100961: NEDD1 (39-J) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
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Rated 5 de 5 de par Great for IF staining. This monoclonal worked great for IF labeling of centrosomes in human HEK293 and in CHO cells at a dilution of 1:500.
Date de publication: 2017-07-14
Rated 4 de 5 de par Nedd1 antibody works well in cultured cells Nedd1 antibody works well in cultured cells use at 1:100 in Methanol fix. Works okay in tissue but not so well in western blot.
Date de publication: 2016-07-27
Rated 3 de 5 de par Positive results in WB with mouse NEDD1 transfected Positive results in WB with mouse NEDD1 transfected 293T whole cell lysates. -SCBT QC
Date de publication: 2015-05-10
Rated 5 de 5 de par Strong Strong, clean bands in western blot using U-2 OS whole cell lysate and HeLa nuclear extract. -SCBT QC
Date de publication: 2015-04-24
Rated 4 de 5 de par Great immunoperoxidase membrane staining Great immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date de publication: 2015-02-14
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