Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NDUFS3 CRISPR/Cas9 KO Plasmid (m): sc-427031
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NDUFS3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NDUFS3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NDUFS3 Antibody (D-4): sc-374282
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NDUFS3 CRISPR/Cas9 KO Plasmid (m) | sc-427031 | 20 µg | $397.00 |
Overview
Ndufs3 encodes NDUFS3, an essential nuclear-encoded subunit of mitochondrial respiratory chain complex I (NADH:ubiquinone oxidoreductase) that supports electron transfer from NADH to ubiquinone and contributes to proton pumping across the inner mitochondrial membrane. Proper NDUFS3 function is required for efficient oxidative phosphorylation, maintenance of mitochondrial membrane potential, and regulation of cellular redox balance. Disruption of complex I components can impair ATP production and elevate reactive oxygen species, influencing metabolic remodeling and stress signaling. In mouse models, Ndufs3 perturbation is relevant for studying mitochondrial dysfunction mechanisms implicated in neuromuscular, cardiac, and neurodegeneration-associated phenotypes.
NDUFS3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ndufs3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ndufs3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ndufs3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NDUFS3 protein expression.
This CRISPR knockout system enables efficient generation of Ndufs3-deficient cell models for investigation of NDUFS3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ndufs3 exon(s) critical for NDUFS3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ndufs3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NDUFS3 CRISPR/Cas9 KO Plasmid (m) and NDUFS3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ndufs3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NDUFS3 HDR Plasmid (m) and NDUFS3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ndufs3 homology arms to support homology-directed repair at defined Ndufs3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.