NDRG1 CRISPR/Cas9 KO Plasmid (m): sc-421841
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NDRG1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NDRG1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NDRG1 Antibody (B-5): sc-398291
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NDRG1 CRISPR/Cas9 KO Plasmid (m) | sc-421841 | 20 µg | $397.00 |
Overview
Ndrg1 encodes NDRG1, a cytoplasmic protein induced by cellular stress and differentiation cues that contributes to regulation of cell growth, lineage commitment, and responses to hypoxia. In mouse cells, NDRG1 has been linked to pathways governing iron homeostasis, vesicular trafficking, and cytoskeletal organization, with context-dependent effects on proliferation and motility programs. NDRG1 is also associated with myelin biology in the peripheral nervous system and has been studied in models of neurodegeneration and demyelinating neuropathies. Altered Ndrg1 expression has further been investigated in tumor biology and metastasis-related phenotypes, supporting its relevance in signaling and stress-adaptation studies.
NDRG1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ndrg1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ndrg1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ndrg1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NDRG1 protein expression.
This CRISPR knockout system enables efficient generation of Ndrg1-deficient cell models for investigation of NDRG1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ndrg1 exon(s) critical for NDRG1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ndrg1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NDRG1 CRISPR/Cas9 KO Plasmid (m) and NDRG1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ndrg1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NDRG1 HDR Plasmid (m) and NDRG1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ndrg1 homology arms to support homology-directed repair at defined Ndrg1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.