Anticorps Nanog (5A10) recommandé pour la détection de Nanog d'origine mouse, rat et human par WB, IP et ELISA

Anticorps Nanog (5A10): sc-134218

Anticorps Nanog (5A10) est évalué 4.9 de 5 de 7.
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Fiches techniques

    • L'anticorps Nanog 5A10 est un monoclonal IgG2a de souris, cité dans 29 publications, fourni en 50 µg/500 µl
    • raised against amino acids 1-154 corresponding to recombinant Nanog of human origin
    • recommandé pour la détection de Nanog d'origine mouse, rat et human par WB, IP et ELISA
    • Voir Nanog (1E6C4): sc-293121 pour d'autres anticorps conjugués Nanog, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Nanog (5A10): sc-134218.
    • m-IgG Fc BP-HRP et m-IgG2a BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps Nanog (5A10) pour des applications de WB. Ces réactifs sont maintenant proposés en kit avec l'anticorps Nanog (5A10) (voir Informations pour la commande ci-dessous).
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Is this Nanog (5A10): sc-134218 monoclonal antibody available directly conjugated to HRP?

Posée par: cjMara
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using Nanog (1E6C4): sc-293121, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/nanog-antibody-1e6c4)
Répondue par: Technical Support
Date de publication: 2017-01-30
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Rated 4 de 5 de par Buon funzionamento Ho provato l' anticorpo primario Nanog (5A10):sc-134218. Lo ho usato per western blot e devo dire che il risultato è stato soddisfacente. Ho usato una concentrazione (1:2000) più bassa di quella suggerita (1:1000) ed ho avuto un buon risultato lo stesso su due membrane su cui avevo corso 30µ e 40 µg di proteine. L’incubazione con il primario è stata fatta ON a 4°C ed ho usato un secondario anti mouse 1:20000. Le bande sono nette e c’è poco aspecifico. Dopo l’incubazione con primario e dopo l’incubazione con secondario ho eseguito 3x10 min di lavaggi con TPBS (PBS+0.1% Tween) ed il blocking è stato fatto per 4 ore con Milk al 5% in TPBS a temperatura ambiente. Ritengo sia un prodotto valido per il mio tipo di ricerca e per il western blot in generale.
Date de publication: 2020-07-21
Rated 5 de 5 de par very pleased That was a very good antibody, diluted 1:1000 in human ovary tissue , we obtained good results.
Date de publication: 2017-05-11
Rated 5 de 5 de par great Clean bands obtained in western blot using human ovary tissue extract.
Date de publication: 2017-04-17
Rated 5 de 5 de par Produced publishable WB data on human and mouse Well characterized with publications in the primary literature for WB in human and mouse samples
Date de publication: 2017-01-11
Rated 5 de 5 de par WB: strong WB: strong, clean band in NIH/3T3 whole cell lysate. -SCBT QC
Date de publication: 2015-03-22
Rated 5 de 5 de par Positive results in WB with transfected lysates Positive results in WB with transfected lysates. -SCBT QC
Date de publication: 2015-02-03
Rated 5 de 5 de par Clean bands in western blot analysis of Nanog Clean bands in western blot analysis of Nanog expression in HeLa and Raji whole cell lysates and human ovary tissue extract. -SCBT QC
Date de publication: 2013-08-25
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