Mucin 20 Double Nickase Plasmid (h): sc-415003-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mucin 20 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mucin 20 Double Nickase Plasmid (h) and Mucin 20 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MUC20. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mucin 20 Double Nickase Plasmid (h) | sc-415003-NIC | 20 µg | $410.00 | |||
Mucin 20 Double Nickase Plasmid (h2) | sc-415003-NIC-2 | 20 µg | $410.00 |
MUC20 encodes mucin 20, a membrane-associated mucin expressed at epithelial surfaces where it contributes to glycocalyx structure and modulation of cell–cell and cell–matrix interactions. Mucin 20 has been implicated in regulation of receptor tyrosine kinase signaling, including modulation of MET/HGF pathway output, influencing downstream MAPK/ERK and PI3K/AKT signaling dynamics. Through effects on adhesion, migration, and barrier-associated phenotypes, MUC20 is relevant to studies of epithelial differentiation and tissue remodeling. Altered MUC20 expression has been reported across multiple disease contexts, supporting its use as a molecular handle to interrogate signaling rewiring and epithelial pathobiology in experimental models.
Mucin 20 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MUC20 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MUC20. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MUC20 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MUC20-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.