MORF4L1 Double Nickase Plasmid (m): sc-423346-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MORF4L1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MORF4L1 Double Nickase Plasmid (m) and MORF4L1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Morf4l1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MORF4L1 Antibody (E-8): sc-514877
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MORF4L1 Double Nickase Plasmid (m) | sc-423346-NIC | 20 µg | $410.00 | |||
MORF4L1 Double Nickase Plasmid (m2) | sc-423346-NIC-2 | 20 µg | $410.00 |
Morf4l1 encodes MORF4L1, a nuclear chromatin-associated factor that participates in transcriptional control by modulating histone acetylation dynamics and nucleosome organization. MORF4L1 functions within complexes linked to the NuA4/TIP60 histone acetyltransferase axis, supporting processes such as DNA damage response signaling, chromatin remodeling, and cell-cycle–coupled gene expression programs. Through these roles, MORF4L1 is relevant to studies of genome stability, epigenetic regulation, and stress-responsive transcriptional networks in mouse cells. Dysregulation of MORF4L1-associated pathways is frequently examined in the context of altered proliferation, differentiation, and oncogenic chromatin states, providing a mechanistic entry point for disease-relevant biology without implying clinical outcomes.
MORF4L1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Morf4l1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Morf4l1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Morf4l1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Morf4l1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.