Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MISR II CRISPR/Cas9 KO Plasmid (h): sc-402523
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MISR II CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MISR II genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MISR II Antibody (D-9): sc-377413
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MISR II CRISPR/Cas9 KO Plasmid (h) | sc-402523 | 20 µg | $397.00 |
Overview
AMHR2 encodes the human anti-Müllerian hormone receptor type 2 (MISR II), a transmembrane serine/threonine kinase that binds AMH and initiates signaling via SMAD1/5/8 to regulate developmental programs in the Müllerian duct and gonadal differentiation. Receptor activation coordinates transcriptional responses involved in cell fate decisions, tissue remodeling, and reproductive tract maturation, integrating with broader TGF-β/BMP pathway dynamics. Dysregulated AMH–AMHR2 signaling has been associated with disorders of sexual development and reproductive endocrinology phenotypes, and altered MISR II expression has been investigated in ovarian-derived and other Müllerian lineage tumor contexts. As a pathway node, AMHR2 is useful for probing ligand-dependent receptor signaling, SMAD-mediated gene regulation, and context-specific growth and differentiation responses.
MISR II CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AMHR2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AMHR2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AMHR2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MISR II protein expression.
This CRISPR knockout system enables efficient generation of AMHR2-deficient cell models for investigation of MISR II signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AMHR2 exon(s) critical for MISR II function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AMHR2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MISR II CRISPR/Cas9 KO Plasmid (h) and MISR II CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AMHR2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MISR II HDR Plasmid (h) and MISR II HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AMHR2 homology arms to support homology-directed repair at defined AMHR2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.