Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LSD1 Double Nickase Plasmid (h): sc-401294-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LSD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LSD1 Double Nickase Plasmid (h) and LSD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KDM1A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LSD1 Antibody (B-9): sc-271720
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LSD1 Double Nickase Plasmid (h) | sc-401294-NIC | 20 µg | $410.00 | |||
LSD1 Double Nickase Plasmid (h2) | sc-401294-NIC-2 | 20 µg | $410.00 |
KDM1A encodes lysine-specific demethylase 1 (LSD1), a FAD-dependent amine oxidase that removes mono- and di-methyl marks from histone H3 (primarily H3K4 and context-dependently H3K9) to modulate chromatin accessibility and transcriptional programs. LSD1 functions within corepressor and chromatin remodeling assemblies such as CoREST and NuRD, linking histone demethylation to DNA methylation, enhancer regulation, and lineage-specific gene expression. Through these epigenetic mechanisms, LSD1 influences cell-cycle progression, differentiation, and maintenance of stem-like states. Dysregulated KDM1A activity and altered LSD1 complex composition have been associated with aberrant transcriptional control in multiple cancers and other disorders involving epigenetic misregulation.
LSD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KDM1A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KDM1A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KDM1A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KDM1A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.