Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
L-Selectin/CD62L/SELL CRISPR/Cas9 KO Plasmid (m): sc-422872
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- L-Selectin/CD62L/SELL CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the L-Selectin/CD62L/SELL genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: L-Selectin/CD62L/SELL Antibody (lam1-116): sc-13505
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
L-Selectin/CD62L/SELL CRISPR/Cas9 KO Plasmid (m) | sc-422872 | 20 µg | $397.00 |
Overview
Sell encodes L-selectin (CD62L), a leukocyte adhesion receptor that mediates lymphocyte homing and leukocyte rolling on high endothelial venules through binding to peripheral node addressins and other sulfated, sialylated glycan ligands. In mouse immune cells, CD62L shedding and trafficking coordinate transitions between naïve, central memory, and activated phenotypes, linking adhesion dynamics to chemokine-driven migration and immune surveillance. SELL signaling and ectodomain cleavage intersect with inflammatory cascades and endothelial activation, shaping leukocyte recruitment during tissue injury and infection. Altered L-selectin-dependent trafficking is commonly studied in models of autoimmunity, chronic inflammation, and tumor immunology where cell localization and priming influence disease phenotypes.
L-Selectin/CD62L/SELL CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sell gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sell together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sell open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish L-Selectin/CD62L/SELL protein expression.
This CRISPR knockout system enables efficient generation of Sell-deficient cell models for investigation of L-Selectin/CD62L/SELL signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sell exon(s) critical for L-Selectin/CD62L/SELL function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sell genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by L-Selectin/CD62L/SELL CRISPR/Cas9 KO Plasmid (m) and L-Selectin/CD62L/SELL CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sell locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by L-Selectin/CD62L/SELL HDR Plasmid (m) and L-Selectin/CD62L/SELL HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sell homology arms to support homology-directed repair at defined Sell target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.