KCNQ3 CRISPR/Cas9 KO Plasmid (h): sc-403544
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KCNQ3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KCNQ3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KCNQ3 CRISPR/Cas9 KO Plasmid (h) | sc-403544 | 20 µg | $397.00 |
Overview
KCNQ3 encodes the Kv7.3 subunit of voltage-gated potassium channels that carry the neuronal M-current, a key regulator of membrane excitability and spike-frequency adaptation. By shaping subthreshold conductances, KCNQ3 participates in pathways controlling action potential threshold, synaptic integration, and network oscillations, often in concert with KCNQ2-containing channel complexes. Disruption of KCNQ3-mediated currents alters neuronal firing dynamics and has been associated with neurodevelopmental and epileptic phenotypes, making it relevant to studies of cortical circuit function. KCNQ3 is also used as a functional readout target in ion channel pharmacology and electrophysiology workflows that interrogate excitability-related signaling.
KCNQ3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KCNQ3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KCNQ3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KCNQ3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KCNQ3 protein expression.
This CRISPR knockout system enables efficient generation of KCNQ3-deficient cell models for investigation of KCNQ3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KCNQ3 exon(s) critical for KCNQ3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KCNQ3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KCNQ3 CRISPR/Cas9 KO Plasmid (h) and KCNQ3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KCNQ3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KCNQ3 HDR Plasmid (h) and KCNQ3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KCNQ3 homology arms to support homology-directed repair at defined KCNQ3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.