Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Jagged1 Double Nickase Plasmid (h): sc-400208-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Jagged1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Jagged1 Double Nickase Plasmid (h) and Jagged1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting JAG1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Jagged1 Antibody (E-12): sc-390177
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Jagged1 Double Nickase Plasmid (h) | sc-400208-NIC | 20 µg | $410.00 | |||
Jagged1 Double Nickase Plasmid (h2) | sc-400208-NIC-2 | 20 µg | $410.00 |
JAG1 encodes Jagged1, a transmembrane ligand that activates Notch receptors to regulate juxtacrine signaling controlling cell fate specification, stem/progenitor maintenance, and tissue patterning. Jagged1-driven Notch signaling influences transcriptional programs via RBPJ/CSL and downstream targets such as HES and HEY family genes, shaping processes including angiogenesis, epithelial–mesenchymal interactions, and biliary and cardiovascular development. Dysregulated JAG1/Notch activity is implicated in congenital developmental disorders including Alagille syndrome and contributes to context-dependent tumor biology by altering differentiation states and microenvironmental signaling.
Jagged1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the JAG1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within JAG1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt JAG1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of JAG1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.