IRX1 CRISPR/Cas9 KO Plasmid (m): sc-421153
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IRX1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IRX1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRX1 CRISPR/Cas9 KO Plasmid (m) | sc-421153 | 20 µg | $397.00 |
Overview
Irx1 encodes the Iroquois homeobox transcription factor IRX1, a nuclear DNA-binding regulator that patterns embryonic tissues and guides lineage specification. In mouse development, IRX1 contributes to transcriptional programs governing morphogenesis, cell fate decisions, and spatial organization of organ primordia, integrating with broader homeobox-driven regulatory networks. Dysregulated IRX1 expression or altered regulatory control has been associated with developmental abnormalities and has been studied in the context of aberrant differentiation states relevant to cancer biology. As a transcriptional node, IRX1 is commonly investigated for its downstream gene targets, chromatin-state dependencies, and cross-talk with signaling pathways that shape tissue identity.
IRX1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Irx1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Irx1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Irx1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IRX1 protein expression.
This CRISPR knockout system enables efficient generation of Irx1-deficient cell models for investigation of IRX1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Irx1 exon(s) critical for IRX1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Irx1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IRX1 CRISPR/Cas9 KO Plasmid (m) and IRX1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Irx1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IRX1 HDR Plasmid (m) and IRX1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Irx1 homology arms to support homology-directed repair at defined Irx1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.