Integrin αV/ITGAV/CD51 Double Nickase Plasmid (m): sc-421169-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin αV/ITGAV/CD51 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin αV/ITGAV/CD51 Double Nickase Plasmid (m) and Integrin αV/ITGAV/CD51 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Itgav. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin αV/ITGAV/CD51 Antibody (H-2): sc-376156
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin αV/ITGAV/CD51 Double Nickase Plasmid (m) | sc-421169-NIC | 20 µg | $410.00 |
Itgav encodes integrin αV (ITGAV/CD51), an α subunit that pairs with multiple β integrins to form receptors for extracellular matrix ligands containing RGD motifs, including vitronectin, fibronectin, and osteopontin. Through focal adhesion assembly and crosstalk with FAK/Src, PI3K–AKT, and MAPK signaling, integrin αV regulates cell adhesion, migration, survival, and mechanotransduction, and it can modulate latent TGF-β activation in matrix-rich microenvironments. In mouse tissues, ITGAV contributes to angiogenesis, immune cell trafficking, wound repair, and osteoclast function, linking extracellular matrix remodeling to cell-state decisions. Dysregulated αV-containing integrin signaling is widely studied in fibrosis, inflammation, and tumor-associated stromal interactions, where altered adhesion and matrix signaling can shape invasive and pro-survival phenotypes.
Integrin αV/ITGAV/CD51 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Itgav locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Itgav. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Itgav function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Itgav-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.