Integrin α2/ITGA2/CD49b Double Nickase Plasmid (m): sc-421159-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin α2/ITGA2/CD49b Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin α2/ITGA2/CD49b Double Nickase Plasmid (m) and Integrin α2/ITGA2/CD49b Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Itga2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin α2/ITGA2/CD49b Antibody (C-9): sc-74466
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin α2/ITGA2/CD49b Double Nickase Plasmid (m) | sc-421159-NIC | 20 µg | $410.00 | |||
Integrin α2/ITGA2/CD49b Double Nickase Plasmid (m2) | sc-421159-NIC-2 | 20 µg | $410.00 |
Mouse Itga2 encodes integrin α2 (ITGA2/CD49b), an α subunit that heterodimerizes with integrin β1 to form a collagen- and laminin-binding receptor that links the extracellular matrix to the actin cytoskeleton. Through outside-in and inside-out signaling, ITGA2 modulates focal adhesion assembly and downstream pathways including FAK/Src, PI3K–AKT, and MAPK, shaping cell adhesion, migration, survival, and mechanotransduction. ITGA2 also contributes to platelet and immune cell interactions with collagen-rich matrices, influencing hemostatic and inflammatory responses. Dysregulated integrin α2 signaling has been associated with altered tissue remodeling and invasive phenotypes in diverse disease models, making Itga2 a useful target for dissecting cell–matrix crosstalk.
Integrin α2/ITGA2/CD49b Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Itga2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Itga2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Itga2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Itga2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.