Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IL-1ra Double Nickase Plasmid (h): sc-401313-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-1ra Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-1ra Double Nickase Plasmid (h) and IL-1ra Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL1RN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-1ra Antibody (A-4): sc-374084
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-1ra Double Nickase Plasmid (h) | sc-401313-NIC | 20 µg | $410.00 | |||
IL-1ra Double Nickase Plasmid (h2) | sc-401313-NIC-2 | 20 µg | $410.00 |
IL1RN encodes interleukin-1 receptor antagonist (IL-1ra), a secreted cytokine that competitively inhibits IL-1α and IL-1β binding to IL-1 receptor type I, thereby limiting downstream MyD88-dependent signaling. By dampening NF-κB and MAPK pathway activation, IL-1ra modulates transcriptional programs controlling cytokine production, leukocyte recruitment, and acute-phase inflammatory responses. Dysregulated IL1RN expression or function is associated with heightened inflammatory signaling and has been linked to autoinflammatory and autoimmune phenotypes, underscoring its importance in immune homeostasis. In cellular systems, IL1RN provides a tractable node for interrogating IL-1–driven crosstalk with inflammasome activity and broader cytokine network regulation.
IL-1ra Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL1RN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL1RN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL1RN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL1RN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.