Ikaros Lentiviral Activation Particles (h): sc-400530-LAC

Human IKZF1 encodes Ikaros, a zinc-finger transcription factor that binds regulatory DNA elements to control chromatin remodeling and lineage-specific gene expression during hematopoiesis. Ikaros is a central regulator of lymphoid differentiation, influencing B- and T-cell development, immune homeostasis, and cell-cycle and apoptotic programs through broad transcriptional networks. Through interactions with chromatin-modifying complexes, it helps coordinate developmental checkpoints and maintenance of cellular identity. Dysregulation of IKZF1 expression or function is frequently studied in hematologic disease models due to its impact on differentiation state, genomic stability, and transcriptional control.

Ikaros Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient IKZF1 upregulation across a broader range of human cell types.

Ikaros Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the IKZF1 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous Ikaros expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native IKZF1 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.