HuB CRISPR/Cas9 KO Plasmid (h): sc-400169
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HuB CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HuB genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HuB CRISPR/Cas9 KO Plasmid (h) | sc-400169 | 20 µg | $397.00 |
Overview
ELAVL2 (HuB) is a neuron-enriched RNA-binding protein of the ELAV/Hu family that recognizes AU-rich elements to regulate pre-mRNA processing, mRNA stability, localization, and translation. By shaping post-transcriptional gene expression programs, HuB contributes to neuronal differentiation, synaptic development, and activity-dependent plasticity through control of transcripts involved in neurogenesis and cytoskeletal dynamics. ELAVL2-associated RNA regulons intersect with pathways governing cell fate specification and stress responses, and altered expression patterns have been reported in neurodevelopmental and neurodegenerative disease contexts as well as in some nervous system malignancies. These features make ELAVL2 a useful node for dissecting how RNA metabolism influences neuronal identity and dysfunction.
HuB CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ELAVL2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ELAVL2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ELAVL2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HuB protein expression.
This CRISPR knockout system enables efficient generation of ELAVL2-deficient cell models for investigation of HuB signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ELAVL2 exon(s) critical for HuB function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ELAVL2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HuB CRISPR/Cas9 KO Plasmid (h) and HuB CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ELAVL2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HuB HDR Plasmid (h) and HuB HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ELAVL2 homology arms to support homology-directed repair at defined ELAVL2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.