Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSPA8/HSC70 Double Nickase Plasmid (h): sc-418508-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSPA8/HSC70 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSPA8/HSC70 Double Nickase Plasmid (h) and HSPA8/HSC70 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HSPA8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSPA8/HSC70 Antibody (B-6): sc-7298
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSPA8/HSC70 Double Nickase Plasmid (h) | sc-418508-NIC | 20 µg | $410.00 | |||
HSPA8/HSC70 Double Nickase Plasmid (h2) | sc-418508-NIC-2 | 20 µg | $410.00 |
HSPA8 encodes the constitutively expressed chaperone HSC70, a central component of the HSP70 family that binds nascent or stress-denatured polypeptides to promote folding, refolding, and prevention of aggregation. HSC70 participates in ATP-dependent chaperone cycles with co-chaperones such as DNAJs and nucleotide exchange factors, supporting proteostasis across cytosol and organelles. It is also a key regulator of chaperone-mediated autophagy, clathrin uncoating during endocytosis, and protein quality control pathways including ubiquitin–proteasome turnover. Dysregulation of HSPA8/HSC70-linked proteostasis and trafficking has been implicated in neurodegeneration, cancer cell stress tolerance, and infectious disease biology, making it a widely studied node in cellular homeostasis networks.
HSPA8/HSC70 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HSPA8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HSPA8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HSPA8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HSPA8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.