Anticorps hepcidin (56-Z) recommandé pour la détection de hepcidin d'origine human par IF, IHC(P) et ELISA

Anticorps hepcidin (56-Z): sc-100277

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Anticorps hepcidin (56-Z) is rated 4.5 out of 5 by 2.
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Fiches techniques

    • Anti-L'anticorps hepcidin 56-Z est un monoclonal IgG1 (chaîne légère kappa) L'Anticorps hepcidin fourni en 100 µg/ml
    • raised against full-length recombinant protein mapping within amino acids 25-85 of hepcidin of human origin
    • recommandé pour la détection de hepcidin d'origine human par IF, IHC(P) et ELISA
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.

Dear technique support, I have bought the antibody of hepcidin(56-Z): sc-100277, I want to do immunohistochemistry, can you tell me the protocol that you used it to do immunohistochemistry? Thank you very much!

Posée par: thrust han
Thank you for your question. You can find our recommended protocol for IHC applications by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunoperoxidase-staining
Répondue par: Tech Support Europe
Date de publication: 2020-08-28

Is there trial size of hepcidin(56-Z): sc-100277? I want to measure the effect of it firstly.

Posée par: thrust han
Thank you for your question. We do not offer a trial size of this antibody, unfortunately. However, it is recommended and warrantied for detection of hepcidin of human origin by IF, IHC(P) and ELISA, so there is no risk involved if you decide to purchase it.
Répondue par: Tech Support Europe
Date de publication: 2020-08-04

Hi Is this antibody only specific for human or just not tested for other species (e.g. mouse & rat)? In case not tested, is it possible to obtain a trial vial to test the antibody on both rat and mice liver specimens?

Posée par: Bassem
Thank you for your inquiry and your interest in this product. We have not tested the hepcidin Antibody (56-Z): sc-100277 on mouse or rat. Thus we do not recommend nor warrant it for the detection of hepcidin from mouse or rat origin. We do not offer this antibody in a sample size. For further questions please contact our European Support Office in Germany directly. You can reach us by phone at: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com We are happy to assist you.
Répondue par: Technical Support Europe
Date de publication: 2017-12-15

Does it work on mouse hepcidin?

Posée par: Deliang
Thank you for your question. This antibody, sc-100277 Hepcidin (56-Z) has not been tested for mouse and is therefore not recommended nor warrantied to use on mouse samples. It may work, but we can not recommend that it will do so. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Répondue par: Tech Service
Date de publication: 2017-06-06

Can sc-100277: hepcidin (56-Z) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Posée par: Germaine
Thank you for your question. Yes, sc-100277: hepcidin (56-Z) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
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