HARBI1 Double Nickase Plasmid (m): sc-433844-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HARBI1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HARBI1 Double Nickase Plasmid (m) and HARBI1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Harbi1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HARBI1 Double Nickase Plasmid (m) | sc-433844-NIC | 20 µg | $410.00 |
Harbi1 encodes HARBI1, a domesticated transposase-derived protein implicated in genome biology and the regulation of DNA-associated processes. Although its molecular functions remain incompletely defined, HARBI1 has been linked to pathways relevant to DNA repair, chromatin dynamics, and maintenance of genomic stability, making it of interest in studies of cell cycle control and stress responses. In mouse models, perturbation of genome maintenance factors can influence developmental phenotypes and cellular fitness, and HARBI1 is therefore investigated in contexts where altered DNA damage responses or chromatin regulation contribute to disease-relevant mechanisms. Understanding HARBI1 activity supports research into how transposase-derived genes are repurposed to modulate mammalian gene regulation and genome integrity.
HARBI1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Harbi1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Harbi1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Harbi1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Harbi1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.