HAPLN1 Double Nickase Plasmid (h): sc-403874-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HAPLN1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HAPLN1 Double Nickase Plasmid (h) and HAPLN1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HAPLN1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAPLN1 Double Nickase Plasmid (h) | sc-403874-NIC | 20 µg | $410.00 | |||
HAPLN1 Double Nickase Plasmid (h2) | sc-403874-NIC-2 | 20 µg | $410.00 |
HAPLN1 (hyaluronan and proteoglycan link protein 1) encodes an extracellular matrix glycoprotein that stabilizes aggregates of hyaluronan with chondroitin sulfate proteoglycans, supporting matrix assembly, hydration, and tissue biomechanics. HAPLN1 contributes to cell–matrix interactions and ECM organization programs that influence adhesion, migration, and mechanotransduction, particularly in connective tissues and cartilage-like microenvironments. Altered HAPLN1 expression and matrix-linking activity have been associated with dysregulated stromal remodeling, fibrosis-associated pathways, and tumor microenvironment composition, making it a useful node for studying extracellular matrix dynamics in human disease models. As a secreted/ECM-associated factor, HAPLN1 is commonly interrogated in systems biology of matrix turnover, inflammatory remodeling, and metastasis-related niche formation.
HAPLN1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HAPLN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HAPLN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HAPLN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HAPLN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.