Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HAI-2 CRISPR/Cas9 KO Plasmid (m): sc-423129
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HAI-2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HAI-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HAI-2 Antibody (H-9): sc-398119
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAI-2 CRISPR/Cas9 KO Plasmid (m) | sc-423129 | 20 µg | $397.00 |
Overview
Spint2 encodes the serine protease inhibitor HAI-2, a membrane-associated Kunitz-type regulator that constrains pericellular proteolysis by inhibiting type II transmembrane serine proteases such as matriptase and hepsin. By limiting protease-driven activation of growth factors and modulation of extracellular matrix turnover, HAI-2 influences epithelial barrier integrity, tissue remodeling, and cell polarity programs. In mouse systems, altered SPINT2/HAI-2 activity is used to study dysregulated protease–inhibitor balance linked to epithelial dysfunction and aberrant stromal–epithelial signaling. These processes intersect with pathways governing adhesion, migration, and protease-dependent signaling cascades relevant to inflammation and tumor biology modeling.
HAI-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Spint2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Spint2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Spint2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HAI-2 protein expression.
This CRISPR knockout system enables efficient generation of Spint2-deficient cell models for investigation of HAI-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Spint2 exon(s) critical for HAI-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Spint2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HAI-2 CRISPR/Cas9 KO Plasmid (m) and HAI-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Spint2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HAI-2 HDR Plasmid (m) and HAI-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Spint2 homology arms to support homology-directed repair at defined Spint2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.