Grid2ip Antibody (A-4): sc-390952
- Grid2ip Antibody (A-4) is a mouse monoclonal IgG1 κ Grid2ip antibody provided at 200 µg/ml
- raised against amino acids 91-280 mapping near the N-terminus of Grid2ip of mouse origin
- Grid2ip Antibody (A-4) is recommended for detection of Grid2ip of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Grid2ip Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for Grid2ip Antibody (A-4) for WB applications. This reagent is now offered in a bundle with Grid2ip Antibody (A-4) (see ordering information below).
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Grid2ip Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Grid2ip protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Grid2ip (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Grid2ip protein, also known as glutamate receptor, ionotropic, δ2 (Grid2 or GluR-δ2) interacting protein 1 or delphilin, serves as a crucial postsynaptic scaffolding protein that contains one formin homology 2 domain and two PDZ domains, which are essential for protein-protein interactions. Grid2ip is predominantly expressed in Purkinje cells of the cerebellum, where Grid2ip localizes specifically to parallel fiber synapses, facilitating interaction with the C-terminus of GluR-δ2. This interaction links GluR-δ2, a glutamate receptor integral to motor learning, cerebellar wiring, and synaptic plasticity, with various signaling molecules and the actin cytoskeleton. Alternative splicing of Grid2ip gene results in three isoforms: L-delphilin, S-delphilin (or delphilin-α), and delphilin-β, each exhibiting distinct expression patterns and protein interactions. Palmitoylation of delphilin-α enhances GluR-δ2 expression on the cell surface and promotes delphilin-α accumulation in dendritic spines, underscoring this modification′s importance in synaptic function and plasticity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Grid2ip Antibody (A-4) References:
- Delphilin: a novel PDZ and formin homology domain-containing protein that synaptically colocalizes and interacts with glutamate receptor delta 2 subunit. | Miyagi, Y., et al. 2002. J Neurosci. 22: 803-14. PMID: 11826110
- Identification and characterization of human GRID2IP gene and rat Grid2ip gene in silico. | Katoh, M. and Katoh, M. 2003. Int J Mol Med. 12: 1015-9. PMID: 14612983
- Identification and characterization of human DIAPH3 gene in silico. | Katoh, M. and Katoh, M. 2004. Int J Mol Med. 13: 473-8. PMID: 14767582
- Identification and characterization of a novel Delphilin variant with an alternative N-terminus. | Yamashita, T., et al. 2005. Brain Res Mol Brain Res. 141: 83-94. PMID: 16168524
- Characterization of the delta2 glutamate receptor-binding protein delphilin: Splicing variants with differential palmitoylation and an additional PDZ domain. | Matsuda, K., et al. 2006. J Biol Chem. 281: 25577-87. PMID: 16835239
- Binding of glutamate receptor delta2 to its scaffold protein, Delphilin, is regulated by PKA. | Sonoda, T., et al. 2006. Biochem Biophys Res Commun. 350: 748-52. PMID: 17027646
- The extreme C-terminus of GluRdelta2 is essential for induction of long-term depression in cerebellar slices. | Kohda, K., et al. 2007. Eur J Neurosci. 25: 1357-62. PMID: 17425562
- The synaptic scaffolding protein Delphilin interacts with monocarboxylate transporter 2. | Watanabe-Kaneko, K., et al. 2007. Neuroreport. 18: 489-93. PMID: 17496809
- Enhancement of both long-term depression induction and optokinetic response adaptation in mice lacking delphilin. | Takeuchi, T., et al. 2008. PLoS One. 3: e2297. PMID: 18509461
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Grid2ip Antibody (A-4) | sc-390952 | 200 µg/ml | $322.00 | |||
Grid2ip Antibody (A-4): m-IgG1 BP-HRP Bundle | sc-531748 | 200 µg Ab; 20 µg BP | $361.00 | |||
Grid2ip Antibody (A-4) AC | sc-390952 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Grid2ip Antibody (A-4) HRP | sc-390952 HRP | 200 µg/ml | $322.00 | |||
Grid2ip Antibody (A-4) FITC | sc-390952 FITC | 200 µg/ml | $336.00 | |||
Grid2ip Antibody (A-4) PE | sc-390952 PE | 200 µg/ml | $349.00 | |||
Grid2ip Antibody (A-4) Alexa Fluor® 488 | sc-390952 AF488 | 200 µg/ml | $364.00 | |||
Grid2ip Antibody (A-4) Alexa Fluor® 546 | sc-390952 AF546 | 200 µg/ml | $364.00 | |||
Grid2ip Antibody (A-4) Alexa Fluor® 594 | sc-390952 AF594 | 200 µg/ml | $364.00 | |||
Grid2ip Antibody (A-4) Alexa Fluor® 647 | sc-390952 AF647 | 200 µg/ml | $364.00 | |||
Grid2ip Antibody (A-4) Alexa Fluor® 680 | sc-390952 AF680 | 200 µg/ml | $364.00 | |||
Grid2ip Antibody (A-4) Alexa Fluor® 790 | sc-390952 AF790 | 200 µg/ml | $364.00 |