Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GPRC5B Double Nickase Plasmid (h): sc-409271-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPRC5B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPRC5B Double Nickase Plasmid (h) and GPRC5B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPRC5B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPRC5B Antibody (A-8): sc-518246
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPRC5B Double Nickase Plasmid (h) | sc-409271-NIC | 20 µg | $410.00 | |||
GPRC5B Double Nickase Plasmid (h2) | sc-409271-NIC-2 | 20 µg | $410.00 |
GPRC5B (G protein-coupled receptor family C group 5 member B) is an orphan, retinoic acid–inducible GPCR-like protein enriched at the plasma membrane and implicated in modulation of receptor signaling and membrane-proximal adaptor networks. It has been linked to regulation of MAPK/ERK and PI3K/AKT signaling dynamics, influencing cell growth, differentiation, and stress-responsive transcriptional programs. In human tissues, GPRC5B expression correlates with metabolic and inflammatory phenotypes, and altered regulation has been reported in contexts including obesity-associated insulin signaling, neuroinflammation, and tumor biology. As a signaling node, GPRC5B is frequently studied for its effects on pathway crosstalk, receptor trafficking, and downstream transcriptional outputs.
GPRC5B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPRC5B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPRC5B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPRC5B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPRC5B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.