Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
glypican-5 Antibody (F-3): sc-390838. Western blot analysis of glypican-5 expression in untreated K-562 (A), chemically-treated K-562 (B), HCT-116 (C) and HeLa (D) whole cell lysates. β-Actin (C4): sc-47778 used as loading control. Detection reagent used: m-IgG Fc BP-HRP: sc-525409.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
glypican-5 CRISPR/Cas9 KO Plasmid (h): sc-407289
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- glypican-5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the glypican-5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: glypican-5 Antibody (F-3): sc-390838
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
glypican-5 CRISPR/Cas9 KO Plasmid (h) | sc-407289 | 20 µg | $397.00 |