Glucose Transporter Glut2 CRISPR/Cas9 KO Plasmid (h): sc-400347

Overview

SLC2A2 encodes glucose transporter 2 (GLUT2), a facilitative hexose transporter that mediates bidirectional glucose flux across the plasma membrane in a concentration-dependent manner. In human tissues, GLUT2 contributes to nutrient sensing and metabolic homeostasis by supporting glucose uptake and release and influencing downstream pathways linked to glycolysis, gluconeogenesis, and insulin secretion dynamics. Altered SLC2A2 expression or function has been associated with dysregulated glucose handling and metabolic phenotypes, making it relevant for studies of diabetes biology, hepatic glucose output, and pancreatic islet function. Because GLUT2 can also transport other hexoses, perturbation of SLC2A2 impacts broader carbohydrate utilization and cellular energy balance.

Glucose Transporter Glut2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC2A2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC2A2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC2A2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Glucose Transporter Glut2 protein expression.

This CRISPR knockout system enables efficient generation of SLC2A2-deficient cell models for investigation of Glucose Transporter Glut2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting SLC2A2 exon(s) critical for Glucose Transporter Glut2 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple SLC2A2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Glucose Transporter Glut2 CRISPR/Cas9 KO Plasmid (h) and Glucose Transporter Glut2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC2A2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Glucose Transporter Glut2 HDR Plasmid (h) and Glucose Transporter Glut2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC2A2 homology arms to support homology-directed repair at defined SLC2A2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.