GKLF/KLF4 Antibody (C-6): sc-166101

GKLF Antibody (C-6) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 1-180 of human GKLF, also known as Kruppel-like factor 4 (KLF4). Anti-GKLF antibody (C-6) is validated for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), with reactivity specific to human proteins. KLF4 is a zinc finger transcription factor belonging to the Kruppel-like factor family, which regulates processes including cell proliferation, differentiation, and development. KLF4 is predominantly localized in the nucleus, functioning as a transcriptional regulator by binding to specific DNA sequences, where KLF4 activates or represses gene expression depending on the cellular context. This nuclear localization is critical for KLF4′s role in controlling genes essential for maintaining epithelial homeostasis and response to stress. KLF4 is highly expressed in epithelial tissues, such as the gastrointestinal tract and skin, and is involved in growth arrest and cellular differentiation. KLF4 dysregulation has been associated with pathological conditions, including cancer, where KLF4 may act as either a tumor suppressor or oncogene. GKLF (C-6) antibody enables researchers to study the localization, function, and regulatory mechanisms of KLF4 in development, epithelial maintenance, and disease progression.

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GKLF/KLF4 Antibody (C-6) References:

1. The gut-enriched Kruppel-like factor (Kruppel-like factor 4) mediates the transactivating effect of p53 on the p21WAF1/Cip1 promoter.  |  Zhang, W., et al. 2000. J Biol Chem. 275: 18391-8. PMID: 10749849
2. Up-regulation of gut-enriched krüppel-like factor by interferon-gamma in human colon carcinoma cells.  |  Chen, ZY., et al. 2000. FEBS Lett. 477: 67-72. PMID: 10899312
3. Decreased expression of the gut-enriched Krüppel-like factor gene in intestinal adenomas of multiple intestinal neoplasia mice and in colonic adenomas of familial adenomatous polyposis patients.  |  Dang, DT., et al. 2000. FEBS Lett. 476: 203-7. PMID: 10913614
4. Expression of the gut-enriched Krüppel-like factor (Krüppel-like factor 4) gene in the human colon cancer cell line RKO is dependent on CDX2.  |  Dang, DT., et al. 2001. Oncogene. 20: 4884-90. PMID: 11521200
5. Gut-enriched Krüppel-like factor regulates colonic cell growth through APC/beta-catenin pathway.  |  Stone, CD., et al. 2002. FEBS Lett. 530: 147-52. PMID: 12387883
6. Down-regulation of gut-enriched Kruppel-like factor expression in esophageal cancer.  |  Wang, N., et al. 2002. World J Gastroenterol. 8: 966-70. PMID: 12439907
7. Transcriptional regulation of A33 antigen expression by gut-enriched Krüppel-like factor.  |  Mao, Z., et al. 2003. Oncogene. 22: 4434-43. PMID: 12853980
8. The leukocyte integrin gene CD11d is repressed by gut-enriched Kruppel-like factor 4 in myeloid cells.  |  Noti, JD., et al. 2005. J Biol Chem. 280: 3449-57. PMID: 15561714
9. Gut-enriched Krüppel-like factor interaction with Smad3 inhibits myofibroblast differentiation.  |  Hu, B., et al. 2007. Am J Respir Cell Mol Biol. 36: 78-84. PMID: 16858008
10. Identification and characterization of a gene encoding a gut-enriched Krüppel-like factor expressed during growth arrest.  |  Shields, JM., et al. 1996. J Biol Chem. 271: 20009-17. PMID: 8702718

The preferred GKLF/KLF4 antibody is GKLF/KLF4 Antibody (B-8): sc-393462.