Date published: 2025-9-8

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Anticorps GFP (1A5): sc-101536

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Fiches techniques
  • L'anticorps GFP 1A5 est un monoclonal IgG1 de rat, cité dans 29 publications, fourni en 200 µg/ml
  • soulevé contre le recombinant GFP
  • recommandé pour la détection de GFP and GFP fusion proteins par WB, IP, IF et ELISA
  • Nous testons actuellement encore nos anticorps secondaires pour trouver la meilleure protéine de liaison pour cet anticorps primaire GFP (1A5). Veuillez nous contacter si vous avez des questions concernant les réactifs de détection secondaire appropriés.

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

La protéine fluorescente verte (GFP) a été identifiée à l'origine comme une protéine impliquée dans la bioluminescence de la méduse Aequorea victoria. L'ADNc de la GFP produit un produit du gène lorsqu'il est exprimé dans des cellules procaryotes, sans avoir besoin de substrats ou de cofacteurs exogènes, ce qui fait de la GFP un outil utile pour surveiller l'expression des gènes et la localisation des protéines in vivo. Plusieurs mutants de la GFP ont été développés, notamment l'EGFP, qui émet une fluorescence plus intense que la GFP de type sauvage. Leurs maxima d'excitation décalés sont plus favorables à la microscopie à fluorescence et au FACS, ainsi qu'aux applications de double marquage. La GFP est largement utilisée dans les vecteurs d'expression en tant que protéine de fusion, ce qui permet l'expression et le suivi de protéines hétérologues fusionnées à la GFP.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps GFP (1A5)

sc-101536
200 µg/ml
$316.00

Does this antibody(GFP Antibody (1A5)) works for venus? If not, do you have any idea which GFP antibody here can recognize Venus?

Posée par: Liu M
Thank you for your question. Please contact our Technical Service Department with the specific protein sequence for Venus, and we can perform sequence analysis of our GFP antibody antigens to see if any of them may also detect this protein. We do not have any data or citations for this GFP antibody specifically mentioning reactivity with Venus.
Répondue par: Technical Support
Date de publication: 2020-03-03

Could you tell me which regions or amino acid of the GFP protein is targeted by this antibody?

Posée par: jfoul78
Thank you for your question. GFP Antibody (1A5) is a rat monoclonal raised against recombinant GFP. Unfortunately, the exact sequence used as an immunogen is not known, nor is the epitope recognized by this specific clone. We do offer a broad variety of GFP antibodies where this information is available. For example, our GFP Antibody (C-2): sc-390394 is specific for an epitope mapping between amino acids 167-182 within an internal region of GFP of Aequorea victoria origin. Please contact our Technical Service Department for help in choosing the best antibody for your research needs. Please call us toll-free at (800) 457-3801 option 2, email scbt@scbt.com, or use our live chat.
Répondue par: Technical Support
Date de publication: 2017-02-03

I would like to use GFP Antibody (1A5): sc-101536 for double staining with another mouse monoclonal primary antibody. Is this possible?

Posée par: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-101536 is not available directly conjugated, but we have an alternative GFP antibody (sc-9996) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Répondue par: Technical Support 7
Date de publication: 2017-01-24

I am looking for a GFP antibody that was raised against the amino acid sequence 215-230 (RDHMVL LEFVTAAGIT). Do you have any antibody like this? Thanks!

Posée par: Anette
Thank you for your question. Most of our GFP antibodies were raised against full length recombinant GFP, which includes this portion of the sequence that you have specified. However, the exact epitope recognized by each monoclonal antibody has not been determined. If you would be interested in testing a complimentary sample size of our mouse monoclonal antibody, GFP (B-2): sc-9996, to see if it would work for your purposes, please contact our Technical Service Department. Please call us directly at (800) 457-3801 option 2, email scbt@scbt.com, or use our live chat.
Répondue par: Technical Support
Date de publication: 2017-01-19
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Rated 1 de 5 de par Did not work in texted tissue samplesI got a free sample and tested it according to th eworking lab protocol.There was no signal in the blot.
Date de publication: 2018-04-09
Rated 5 de 5 de par Positive WB and IFAntibody is positive for both westren blot and IF staining
Date de publication: 2017-02-05
Rated 5 de 5 de par Reliable antibodyGood choice if you're looking for a reliable GFP antibody to use in WB and IF.
Date de publication: 2017-01-11
Rated 5 de 5 de par Strong bands at 1:1000 dilutionStrong bands at 1:1000 dilution.
Date de publication: 2015-09-10
Rated 5 de 5 de par Worked well in WBWorked well in WB, no complaints.
Date de publication: 2015-05-27
Rated 5 de 5 de par Good antibody for western blot analysis ofGood antibody for western blot analysis of GFP expression in 293T cell lysate. -SCBT QC
Date de publication: 2015-04-07
Rated 5 de 5 de par Work well for IF staining of COS cellsWork well for IF staining of COS cells. -SCBT QC
Date de publication: 2015-02-01
Rated 5 de 5 de par Generated publishable data on Hela cellsGenerated publishable data on Hela cells with this antibody. -SCBT Publication Review
Date de publication: 2013-11-23
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Anticorps GFP (1A5) est évalué 4.5 de 5 de 8.
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