Date published: 2025-9-8

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Anticorps GFP (15): sc-101525

4.8(12)
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Fiches techniques
  • L'Anticorps GFP (15) est un monoclonal de souris IgG1 κ, cité dans 24 publications, fourni en 200 µg/ml
  • soulevé contre le recombinant GFP
  • recommandé pour la détection de GFP par IF et IHC(P)
  • Nous testons actuellement encore nos anticorps secondaires pour trouver la meilleure protéine de liaison pour cet anticorps primaire GFP (15). Veuillez nous contacter si vous avez des questions concernant les réactifs de détection secondaire appropriés.

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

La protéine fluorescente verte (GFP) a été identifiée à l'origine comme une protéine impliquée dans la bioluminescence de la méduse Aequorea victoria. L'ADNc de la GFP produit un produit du gène lorsqu'il est exprimé dans des cellules procaryotes, sans avoir besoin de substrats ou de cofacteurs exogènes, ce qui fait de la GFP un outil utile pour surveiller l'expression des gènes et la localisation des protéines in vivo. Plusieurs mutants de la GFP ont été développés, notamment l'EGFP, qui émet une fluorescence plus intense que la GFP de type sauvage. Leurs maxima d'excitation décalés sont plus favorables à la microscopie à fluorescence et au FACS, ainsi qu'aux applications de double marquage. La GFP est largement utilisée dans les vecteurs d'expression en tant que protéine de fusion, ce qui permet l'expression et le suivi de protéines hétérologues fusionnées à la GFP.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps GFP (15)

sc-101525
200 µg/ml
$316.00

Is this antibody also recognizing YFP?

Posée par: Adrian31
Thank you for your question,. The antibody does not detect YFP.
Répondue par: BlakeJ
Date de publication: 2023-08-09

You recommended the GFP Antibody (15): sc-101525 for detection of GFP by IHC(P) but I see no picture and cannot find any published data? Could you share your data or help me find published papers. I am OK to test a free sample of the antibody. Thx

Posée par: UCRes
Thank you for your question. We recommend this mouse monoclonal antibody, GFP (15): sc-101525, for detection of GFP by IF and IHC(P) based on publications in the literature. For one reference, please refer to the publication below on PubMed, PMID: 20099318, which is also listed as a product citation on our website (with a link out to PubMed). We are not currently offering a free sample size for this particular antibody. As an alternative, if you would like to test a free sample of GFP Antibody (B-2): sc-9996, please contact our Technical Service Department. This antibody is not currently recommended for IHC(P) because we have not yet tested the antibody with FFPE, however, it also has been published for this application and may be suitable.
Répondue par: Technical Support
Date de publication: 2017-09-11

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with GFP Antibody (15): sc-101525?

Posée par: Trav11
Thank you for your question. We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Répondue par: Technical Support 3
Date de publication: 2017-02-03
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Rated 5 de 5 de par Great antibodyWorked very well with Western blotting and IP. Would highly recommend this antibody.
Date de publication: 2019-05-10
Rated 4 de 5 de par GOODI had this one to test the CONSTRUCT, and it worked well in HEK lysate
Date de publication: 2018-05-14
Rated 5 de 5 de par Really good antibodyGot this as sample.Now ordering it for future use.
Date de publication: 2017-12-16
Rated 5 de 5 de par Good effectGFP used for detect tumor cell, IF of this antibody is obvious.
Date de publication: 2017-09-06
Rated 5 de 5 de par Good Antibody!We used this antibody for IF staining and WB, and it has got great results with a nice clear image.
Date de publication: 2017-09-04
Rated 3 de 5 de par One work, one didn'tfirst one I bought didn't work. Then the tech person sent me two more, same one I picked still not work. the other one he suggested worked.
Date de publication: 2017-03-23
Rated 5 de 5 de par Great IF stainWe used this antibody for IF staining and got great results with a nice clear image
Date de publication: 2017-01-10
Rated 5 de 5 de par This antibody is good for Western BlotI used GFP-tag antibody at a dilution of 1:2000 in PBST for 1 h and is enough for an excellent Western blot signal when the protein from DF-1 cell lysate.
Date de publication: 2016-11-24
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Anticorps GFP (15) est évalué 4.8 de 5 de 12.
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