GCN1L1 CRISPR/Cas9 KO Plasmid (h): sc-411545
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GCN1L1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GCN1L1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCN1L1 CRISPR/Cas9 KO Plasmid (h) | sc-411545 | 20 µg | $397.00 |
Overview
GCN1 (encoding GCN1L1) is a conserved cofactor in the integrated stress response that supports amino acid–sensing control of translation through the GCN2–eIF2α axis. By facilitating activation of eIF2α kinases under nutrient limitation and ribosome-associated stress, GCN1L1 contributes to global translational attenuation and selective induction of stress-adaptive programs such as ATF4-dependent transcription. This regulation links GCN1L1 to proteostasis, oxidative and mitochondrial stress responses, and broader metabolic homeostasis. Dysregulation of translational stress signaling and nutrient-response pathways where GCN1L1 functions has been associated with phenotypes relevant to cancer biology, neurodegeneration, and inflammatory states, supporting mechanistic studies in human cellular models.
GCN1L1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GCN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GCN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GCN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GCN1L1 protein expression.
This CRISPR knockout system enables efficient generation of GCN1-deficient cell models for investigation of GCN1L1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GCN1 exon(s) critical for GCN1L1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GCN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GCN1L1 CRISPR/Cas9 KO Plasmid (h) and GCN1L1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GCN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GCN1L1 HDR Plasmid (h) and GCN1L1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GCN1 homology arms to support homology-directed repair at defined GCN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.