Anticorps G alpha s (12) recommandé pour la détection de Gα s d'origine mouse, rat et human par WB et IP

Anticorps Gα s (12): sc-135914

Anticorps Gα s (12) est évalué 4.7 de 5 de 11.
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Fiches techniques

    • L'Anticorps Gα s (12) est un monoclonal de souris IgG1 (chaîne légère kappa) fourni en 200 µg/ml
    • dirigé contre les acides aminés 11-21 de Gα s d'origine human
    • recommandé pour la détection de Gα s d'origine mouse, rat et human par WB et IP
    • disponible conjugué à l'agarose pour IP; et à l'HRP pour WB, IHC(P) et ELISA
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Gα s (12): sc-135914.

Hi, Is this MAb specific for Gα-s? because the description of MAb for Gα-olf says, it can recognize both forms s and olf and in our study, we need to identify which one of the two is involved, so specificity is very necessary.

Posée par: ASM25
Thank you for your question. The antibody was raised against amino acids 11-21 of G s of human origin. The exact binding region of this antibody within the antigen has not been mapped. This antibody has not been tested experimentally for potential cross-reactivity under denaturing conditions in a Western Blot or native conditions in IP against different proteins. You might want to align the antigen sequence against the protein form of interest in order to determine potential reactivity. based on the antigen sequence we do not expect relevant cross-reactivity against G -olf. Please contact our Technical Service by phone or email or by Live Chat if you have any further questions.
Répondue par: SCBT Heidelberg Support
Date de publication: 2018-11-27

Hi, How can I have a 10ul trial sample of the Gα s Antibody (12): sc-135914 antibody. Thanks

Posée par: Zhong Ding
Thank you for your inquiry. For a limited time, we are offering a free 10 µg sample of this antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Répondue par: Technical Support 15
Date de publication: 2018-02-01

For Western Blot, is it recommended to use denatured or non-denatured conditions with this antibody?

Posée par: TinTin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Répondue par: Technical Support 9
Date de publication: 2017-01-12

Can you recommend a positive control to use with Gα s (12): sc-135914 for Western blot?

Posée par: TinTin
We recommend Jurkat Whole Cell Lysate (sc-2204) or rat or mouse brain tissue extract (sc-2392 or sc-2253) as positive controls as we have Western blot data showing specific detection of Gα s in these samples.
Répondue par: Technical Support 9
Date de publication: 2017-01-12
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Rated 4 de 5 de par Strong Antibody Signal for WB We tried a sample antibody on WB, signal was strong, but we got two bands.
Date de publication: 2017-11-03
Rated 5 de 5 de par no problem The WB of the antibody was detected in the tissues of rat cerebellum, the signals were clear, specific and unique.
Date de publication: 2017-10-26
Rated 5 de 5 de par Worked good This antibody sc-135914 works very well in WB, TBS 5% milk, diluted 1/250.
Date de publication: 2017-08-25
Rated 5 de 5 de par works well It works well in western blotting at dilution of 1:500 analysis Human liver tissue .
Date de publication: 2017-02-25
Rated 3 de 5 de par Moderate specificity Galpha-S (12/sc-135914): Moderate specificity: detects multiple bands from HEK293 lysate, of which one is the correct MW for Galpha-S. Poor sensitivity - may be optimized.
Date de publication: 2017-02-09
Rated 5 de 5 de par Superb western Very nice western blot results; strong signal with low backgrond
Date de publication: 2017-01-31
Rated 5 de 5 de par Great for WB A proven reagent for WB detection. It provides great signal and clear specific bands. It is reactive with mouse, rat, and human and has been published with several times.
Date de publication: 2017-01-30
Rated 5 de 5 de par Various expression levels of the G s protein Various expression levels of the G s protein is demonstrated by Western Blot using Jurkat, HEK293, TT, K562 and MCF-7 whole cell lysates. -SCBT QC
Date de publication: 2015-04-12
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