G_{α i-2} Antibody (L5): sc-13534

Gα i-2 Antibody (L5) is a mouse monoclonal IgG2b kappa light chain antibody raised against the Gα i-2 protein of rat origin, also known as G protein subunit GNAI2. Gα i-2 monoclonal antibody (L5) detects Gα i-2 across multiple species including mouse, rat, human, and bovine, making it suitable for a wide range of applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). The Gα i-2 protein is a crucial member of the heterotrimeric G protein family, which plays an essential role in transmitting signals from cell surface receptors to intracellular effectors. This signal transduction is vital for various cellular processes, as Gα i-2 modulates the activity of key effectors like adenylyl cyclase, thereby influencing the production of second messengers and regulating numerous physiological responses. Functionally, Gα i-2 is part of the Gi class of Gα subunits, which are characterized by their ability to bind and hydrolyze GTP, regulating the activation state of the G protein and its downstream signaling pathways. This regulation is critical for maintaining cellular homeostasis and enabling appropriate responses to extracellular stimuli such as hormones, neurotransmitters, and other signaling molecules. By inhibiting adenylyl cyclase, Gα i-2 plays a pivotal role in decreasing cyclic AMP levels, which is important for processes like the opening of atrial potassium channels and modulation of cardiac function. Gα i-2 (L5) antibody is available in both conjugated and non-conjugated forms, providing researchers with the flexibility to choose the most suitable format for their specific experimental needs.

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G_{α i-2} Antibody (L5) References:

1. Distinct roles for Galpha(i)2 and Gbetagamma in signaling to DNA synthesis and Galpha(i)3 in cellular transformation by dopamine D2S receptor activation in BALB/c 3T3 cells.  |  Ghahremani, MH., et al. 2000. Mol Cell Biol. 20: 1497-506. PMID: 10669727
2. TNF-alpha increases transcription of Galpha(i-2) in human airway smooth muscle cells.  |  Hotta, K., et al. 2000. Am J Physiol Lung Cell Mol Physiol. 279: L319-25. PMID: 10926555
3. Heterologous desensitization of response mediated by selective PKC-dependent phosphorylation of G(i-1) and G(i-2).  |  Murthy, KS., et al. 2000. Am J Physiol Cell Physiol. 279: C925-34. PMID: 11003572
4. Overexpression of wild-type Galpha(i)-2 suppresses beta-adrenergic signaling in cardiac myocytes.  |  Rau, T., et al. 2003. FASEB J. 17: 523-5. PMID: 12631586
5. Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning.  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
6. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
7. Gustducin is a taste-cell-specific G protein closely related to the transducins.  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
8. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
9. Biochemical characterization of three stimulatory GTP-binding proteins. The large and small forms of Gs and the olfactory-specific G-protein, Golf.  |  Jones, DT., et al. 1990. J Biol Chem. 265: 2671-6. PMID: 2105931
10. Small Molecule Inhibitors Targeting Gαi2 Protein Attenuate Migration of Cancer Cells.  |  Caggia, S., et al. 2020. Cancers (Basel). 12: PMID: 32575572
11. Gαi2 Protein Inhibition Blocks Chemotherapy- and Anti-Androgen-Induced Prostate Cancer Cell Migration.  |  Caggia, S., et al. 2024. Cancers (Basel). 16: PMID: 38254786
12. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{α i-2} Antibody (L5) has 80 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{α i-2} Antibody (L5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{α i-2} Antibody (L5) Specifications

Alternate Product Name: G protein subunit GNAI2

Product Clone Name: L5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: Gα i-2

Epitope Species: rat

Product Reactivity/Detection: G_{α i-2}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 80 citations in various scientific publications

Available Conjugates/Forms for G_{α i-2} Antibody (L5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin