Gα i-1/GNAI1 Antibody (SPM397): sc-56536

Gα i-1 Antibody (SPM397) is a mouse monoclonal IgG2b antibody that detects Gα i-1 in mouse, rat, human, and bovine samples through western blotting (WB) and immunoprecipitation (IP) applications. Gα i-1 is a crucial member of the Gi class of G protein alpha subunits, which play a significant role in cellular signaling by inhibiting adenylate cyclase activity and thereby reducing cyclic AMP levels in response to various extracellular signals. This modulation of cyclic AMP is vital for regulating numerous physiological processes, including cardiac function, neurotransmitter release, and hormone signaling. Gα i-1 is primarily located in the plasma membrane, where anti-Gα i-1 antibody (SPM397) can detect interactions with G protein-coupled receptors (GPCRs) that transduce signals from outside the cell to the interior, influencing downstream effectors and cellular responses. Gα i-1′s ability to bind and hydrolyze GTP is essential for function, allowing timely activation and deactivation of signaling pathways, ensuring cellular responses are appropriately regulated. Gα i-1′s interactions with specific receptors and downstream effectors highlight importance in maintaining cellular homeostasis and responding to environmental changes, making Gα i-1 (SPM397) monoclonal antibody a critical tool for research in pharmacology and neurobiology.

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Gα i-1/GNAI1 Antibody (SPM397) References:

1. The myristoylated amino terminus of Galpha(i)(1) plays a critical role in the structure and function of Galpha(i)(1) subunits in solution.  |  Preininger, AM., et al. 2003. Biochemistry. 42: 7931-41. PMID: 12834345
2. Human guanylate binding protein-1 (hGBP-1) characterizes and establishes a non-angiogenic endothelial cell activation phenotype in inflammatory diseases.  |  Naschberger, E., et al. 2005. Adv Enzyme Regul. 45: 215-27. PMID: 16005050
3. Phytoestrogens regulate mRNA and protein levels of guanine nucleotide-binding protein, beta-1 subunit (GNB1) in MCF-7 cells.  |  Naragoni, S., et al. 2009. J Cell Physiol. 219: 584-94. PMID: 19170076
4. Nucleobindin 1 is a calcium-regulated guanine nucleotide dissociation inhibitor of G{alpha}i1.  |  Kapoor, N., et al. 2010. J Biol Chem. 285: 31647-60. PMID: 20679342
5. A novel guanine nucleotide-binding protein coupled to the alpha 1-adrenergic receptor. II. Purification, characterization, and reconstitution.  |  Im, MJ., et al. 1990. J Biol Chem. 265: 18952-60. PMID: 2172240
6. Rad and Rem are non-canonical G-proteins with respect to the regulatory role of guanine nucleotide binding in Ca(V)1.2 channel regulation.  |  Chang, DD. and Colecraft, HM. 2015. J Physiol. 593: 5075-90. PMID: 26426338
7. Guanine Nucleotide-Binding Protein G(i) Subunit Alpha 2 Exacerbates NASH Progression by Regulating Peroxiredoxin 1-Related Inflammation and Lipophagy.  |  Zhang, Z., et al. 2021. Hepatology. 74: 3110-3126. PMID: 34322898
8. Puerarin attenuates diabetic kidney injury through interaction with Guanidine nucleotide-binding protein Gi subunit alpha-1 (Gnai1) subunit.  |  Zhu, Q., et al. 2022. J Cell Mol Med. 26: 3816-3827. PMID: 35678269
9. Hepatic regulator of G protein signaling 14 ameliorates NAFLD through activating cAMP-AMPK signaling by targeting Giα1/3.  |  Wang, J., et al. 2024. Mol Metab. 80: 101882. PMID: 38237897
10. Guanylate-Binding Protein 1 (GBP1) Enhances IFN-α Mediated Antiviral Activity against Hepatitis B Virus Infection.  |  Li, Y., et al. 2024. Pol J Microbiol. 73: 217-235. PMID: 38905278

The preferred G_{α i-1}/GNAI1 antibody is G_{α i-1}/GNAI1 Antibody (R4): sc-13533.