G530011O06Rik Double Nickase Plasmid (m): sc-437289-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- G530011O06Rik Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- G530011O06Rik Double Nickase Plasmid (m) and G530011O06Rik Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting G530011O06Rik. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
G530011O06Rik Double Nickase Plasmid (m) | sc-437289-NIC | 20 µg | $410.00 | |||
G530011O06Rik Double Nickase Plasmid (m2) | sc-437289-NIC-2 | 20 µg | $410.00 |
Mouse G530011O06Rik encodes a largely uncharacterized protein with limited functional annotation, making it a useful target for systematic gene function discovery. Expression patterns and predicted features suggest potential roles in fundamental cellular processes such as regulation of gene expression, RNA metabolism, or protein homeostasis, though definitive pathway placement remains to be established. Perturbation of poorly annotated loci like G530011O06Rik can reveal context-dependent phenotypes linked to cell cycle control, stress responses, or lineage specification. As a result, G530011O06Rik is relevant for exploratory studies that connect genotype to molecular and cellular phenotypes in disease-adjacent models without presupposing a specific mechanism.
G530011O06Rik Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the G530011O06Rik locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within G530011O06Rik. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt G530011O06Rik function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of G530011O06Rik-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.