Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FRRS1L CRISPR/Cas9 KO Plasmid (h): sc-407611
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FRRS1L CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FRRS1L genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FRRS1L Antibody (H-8): sc-398692
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FRRS1L CRISPR/Cas9 KO Plasmid (h) | sc-407611 | 20 µg | $397.00 |
Overview
FRRS1L (ferric chelate reductase 1 like) encodes an endoplasmic reticulum–associated protein implicated in the biogenesis and functional regulation of AMPA-type glutamate receptors, influencing excitatory synaptic transmission and neuronal network activity. FRRS1L participates in processes that govern receptor assembly, trafficking, and surface expression, thereby impacting synaptic plasticity–related signaling pathways. Disruption of FRRS1L function has been linked to neurodevelopmental and epileptic phenotypes, making it relevant for studying mechanisms of synaptopathy and activity-dependent neuronal dysfunction. Its neuronal enrichment and role in glutamatergic signaling support applications in modeling circuit-level consequences of altered receptor homeostasis.
FRRS1L CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FRRS1L gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FRRS1L together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FRRS1L open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FRRS1L protein expression.
This CRISPR knockout system enables efficient generation of FRRS1L-deficient cell models for investigation of FRRS1L signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FRRS1L exon(s) critical for FRRS1L function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FRRS1L genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FRRS1L CRISPR/Cas9 KO Plasmid (h) and FRRS1L CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FRRS1L locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FRRS1L HDR Plasmid (h) and FRRS1L HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FRRS1L homology arms to support homology-directed repair at defined FRRS1L target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.