frizzled-10 CRISPR/Cas9 KO Plasmid (h): sc-406690
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- frizzled-10 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the frizzled-10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
frizzled-10 CRISPR/Cas9 KO Plasmid (h) | sc-406690 | 20 µg | $397.00 |
Overview
FZD10 encodes frizzled-10, a seven-transmembrane WNT receptor that regulates signal transduction through canonical β-catenin–dependent transcription and non-canonical pathways controlling planar cell polarity and calcium flux. By shaping receptor–ligand interactions at the cell surface, frizzled-10 influences developmental patterning, epithelial–mesenchymal programs, and tissue homeostasis. Dysregulated FZD10 activity and altered WNT/Frizzled signaling are frequently studied in the context of oncogenic signaling networks, tumor cell invasion, and aberrant differentiation states. FZD10 is also used as a pathway node for dissecting crosstalk between WNT cues and growth factor–responsive transcriptional programs.
frizzled-10 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FZD10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FZD10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FZD10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish frizzled-10 protein expression.
This CRISPR knockout system enables efficient generation of FZD10-deficient cell models for investigation of frizzled-10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FZD10 exon(s) critical for frizzled-10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FZD10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by frizzled-10 CRISPR/Cas9 KO Plasmid (h) and frizzled-10 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FZD10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by frizzled-10 HDR Plasmid (h) and frizzled-10 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FZD10 homology arms to support homology-directed repair at defined FZD10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.