Anticorps FPR2 (GM1D6) recommandé pour la détection de FPR2 transiently expressed on the cell surface of transfected BOSC cells. d'origine human par WB, IP et FCM

Anticorps FPR2 (GM1D6): sc-57141

Anticorps FPR2 (GM1D6) est évalué 4.6 de 5 de 5.
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Fiches techniques

    • Anti-L'anticorps FPR2 GM1D6 est un monoclonal IgG1 (chaîne légère kappa) L'Anticorps FPR2 fourni en 200 µg/ml
    • dirigé contre FPR2 d'origine human
    • L'Anticorps FPR2 (GM1D6) est recommandé pour la détection de FPR2 transiently expressed on the cell surface of transfected BOSC cells. d'origine human par WB, IP et FCM
    • Anti-L'Anticorps FPR2 (GM1D6) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de FPR2 (GM1D6): sc-57141.
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Boa noite, adquirimos um anticorpo monoclonal FPR2 SC-57141 GM1D6 LOTE #E0217, queremos saber se ele é conjugado a fluoresceína.

Posée par: Maurício420
Thank you for your question. The antibody with catalog number: sc-57141, lot# E0217, is the unconjugated antibody. We do offer this antibody directly conjugated to fluorescein, and the catalog number is sc-57141 FITC.
Répondue par: Technical Support
Date de publication: 2019-08-19

Good nigth, we got a monoclonal antibody FPR2 SC-57141 GM1D6 LOT # E0217, we want to know if it is conjugated to fluorescein.

Posée par: Maurício420
Thank you for your question. That specific catalog and lot number is not conjugated to fluorescein (FITC). The FITC conjugated antibody has the catalog number: sc-57141 FITC.
Répondue par: Technical Support
Date de publication: 2019-06-15

Can FPR2 (GM1D6): sc-57141 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Posée par: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, FPR2 (GM1D6): sc-57141 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 4 de 5 de par Quite good antibody We tested FPR2 by western blotting analysis. 60 micrograms of mouse brain membrane lysates were resolved on 10% SDS-PAGE, and incubated over night with monoclonal FPR2 antibody (1:1000). Secondary antibody utilized was anti-mouse sc-2005 antibody (1:5000). FPR2 signal was detected by chemidoc (Biorad) and the signal (not fully clear) was reached after medium exposition.
Date de publication: 2017-07-25
Rated 5 de 5 de par Reducing or Non-reducing! Great detection of FPR2 in Western Blot under both reducing and non-reducing conditions!
Date de publication: 2017-01-17
Rated 4 de 5 de par The antibody worked pretty good on freshly The antibody worked pretty good on freshly isolated human monocytes and granulocytes, when blocked with 5% heat-inactivated FCS. At a dilution of 1/1000 I observed clear stainings with minimal background and no signal for the isotype control. Tests for cross reactivity in HEK cells expressing hFPR1 or hFPR3 were negative. For high-quality pictures cell had to be fixated with 3% methanol-free paraformaldehyde. In summary the antibody works quite good with good specificity so far.
Date de publication: 2016-04-13
Rated 5 de 5 de par Good specificity in WB in AML Good specificity in WB in AML-193 whole cell lysate. -SCBT QC
Date de publication: 2015-06-14
Rated 5 de 5 de par Robust signal in WB in human PBL and AML Robust signal in WB in human PBL and AML-193 whole cell lysates. -SCBT QC
Date de publication: 2014-07-25
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