Date published: 2025-9-7

00800 4573 8000

SCBT Portrait Logo
Seach Input

Anticorps FOXM1 (G-5): sc-376471

4.0(28)
Écrire une critiquePoser une question

Fiches techniques
  • L'anticorps FOXM1 G-5 est un monoclonal IgG2b κ L'Anticorps FOXM1, cité dans 64 publications, fourni en 200 µg/ml
  • spécifique d'un épitope situé entre les acides aminés 734-763 au niveau C-terminus de FOXM1 d'origine human
  • L'Anticorps FOXM1 (G-5) est recommandé pour la détection de all isoforms of FOXM1 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
  • Anti-L'Anticorps FOXM1 (G-5) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
  • Réactif TransCruz pour une utilisation en Gel Supershift et ChIP (sc-376471 X, 200 µg/0.1 ml)
  • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de FOXM1 (G-5): sc-376471.
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP et m-IgGκ BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps FOXM1 (G-5) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps FOXM1 (G-5) (voir Informations pour la commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

L'anticorps FOXM1 (G-5) est un anticorps monoclonal de souris IgG2b κ FOXM1 (également appelé anticorps HNF3, anticorps FKHL16, anticorps forkhead box M1) qui détecte la protéine FOXM1 de souris, de rat et d'origine humaine par WB, IP, IF, IHC(P) et ELISA. L'anticorps FOXM1 (G-5) est disponible sous forme d'anticorps anti-FOXM1 non conjugué, ainsi que sous plusieurs formes conjuguées d'anticorps anti-FOXM1, y compris agarose, HRP, PE, FITC et plusieurs conjugués Alexa Fluor®. La famille Fox des facteurs de transcription est un grand groupe de protéines qui partagent un domaine commun de liaison à l'ADN appelé hélice ailée ou domaine tête de fourche. FOXM1, également connu sous le nom de FKHL16, MPP2 ou Trident, est principalement exprimé dans les cellules en prolifération. Le gène codant pour FOXM1 humain est localisé sur le chromosome 12p13. L'élément de transcription qui limite l'expression de FOXM1 aux cellules prolifératives est situé 300 pb en amont du codon de départ. FOXM1 est plus abondant dans le thymus, les testicules, l'intestin grêle et le côlon. L'épissage alternatif génère les isoformes FOXM1A et FOXM1B qui contiennent des régions PEST impliquées dans la dégradation rapide des protéines. Une diminution de l'expression de FOXM1 est associée à des défauts de prolifération cellulaire liés à l'âge. Inversement, une augmentation de l'expression de FOXM1B dans le foie de souris transgéniques âgées rétablit le taux de réplication de l'ADN hépatocytaire au niveau du taux plus élevé présent dans les foies jeunes. FOXM1B active la transcription de la cycline B1, de la cycline D1 et de Cdc25B.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Alexa Fluor® est une marque déposée de Molecular Probes Inc., OR., USA

LI-COR® et Odyssey® sont marques déposées de LI-COR Biosciences

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps FOXM1 (G-5)

sc-376471
200 µg/ml
$316.00

FOXM1 (G-5): m-IgG Fc BP-HRP Kit

sc-529873
200 µg Ab; 10 µg BP
$354.00

FOXM1 (G-5): m-IgGκ BP-HRP Kit

sc-522963
200 µg Ab, 40 µg BP
$354.00

FOXM1 (G-5): m-IgG2b BP-HRP Kit

sc-550146
200 µg Ab; 10 µg BP
$354.00

Anticorps FOXM1 (G-5) X

sc-376471 X
200 µg/0.1 ml
$316.00

Anticorps FOXM1 (G-5) AC

sc-376471 AC
500 µg/ml, 25% agarose
$416.00

Anticorps FOXM1 (G-5) HRP

sc-376471 HRP
200 µg/ml
$316.00

Anticorps FOXM1 (G-5) FITC

sc-376471 FITC
200 µg/ml
$330.00

Anticorps FOXM1 (G-5) PE

sc-376471 PE
200 µg/ml
$343.00

Anticorps FOXM1 (G-5) Alexa Fluor® 488

sc-376471 AF488
200 µg/ml
$357.00

Anticorps FOXM1 (G-5) Alexa Fluor® 546

sc-376471 AF546
200 µg/ml
$357.00

Anticorps FOXM1 (G-5) Alexa Fluor® 594

sc-376471 AF594
200 µg/ml
$357.00

Anticorps FOXM1 (G-5) Alexa Fluor® 647

sc-376471 AF647
200 µg/ml
$357.00

Anticorps FOXM1 (G-5) Alexa Fluor® 680

sc-376471 AF680
200 µg/ml
$357.00

Anticorps FOXM1 (G-5) Alexa Fluor® 790

sc-376471 AF790
200 µg/ml
$357.00

FOXM1 (G-5) peptide neutralisant

sc-376471 P
100 µg/0.5 ml
$68.00

FOXM1 G-5 is mouse monoclonal IgG2b. I would like to know if it can be used with conventional secondary anti-mouse IgG reagents.

Posée par: Gemo
Thank you for your question. Yes, you can use a conventional anti-mouse IgG secondary antibody for detection of this primary antibody. For more specific results, we do recommend using an isotype-specific secondary antibody (anti-mouse IgG2b), or our mouse IgGκ binding protein, e.g., m-IgGκ BP-HRP: sc-516102.
Répondue par: Technical Support
Date de publication: 2020-03-09

Cantidad de anticuerpo FOXM1(G5) X TransCruz sc-376471 recomendado para usar en ChIP.

Posée par: femili12
Gracias por sus preguntas. Recomendamos el uso de 100-500 μg de sobrenadante y 0,1-1 μl de reactivo TransCruz (0,2-2 μg). Para obtener más información, haga clic en este enlace de nuestro protocolo ChIP: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays Thank you for your questions. We recommend using 100–500 µg supernatant and 0.1-1 µl TransCruz reagent (0.2–2 µg). For further information, please click on this link to our ChIP protocol: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays
Répondue par: Technical Support
Date de publication: 2018-12-31

Which type of FOXM1  give good results for IHC on parrafin bock,human tissue breast cancer

Posée par: Madyan
Thank you for your question. We would recommend using our mouse monoclonal antibody, FOXM1 (G-5): sc-376471. We have several pieces of IHC(P) data in human tissue using this antibody on our website.
Répondue par: Technical Support
Date de publication: 2017-08-22

Does the FOXM1 Antibody (G-5): sc-376471 detect isoform “C” of the FOXM1 target?

Posée par: Trav11
Thank you for your question. The FOXM1 Antibody (G-5): sc-376471 will detect all isoforms (A, B, & C) for the FOXM1 protein target. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Répondue par: Technical Support 15
Date de publication: 2017-01-16

Does FOXM1 Antibody (G-5): sc-376471 react with Zebrafish samples?

Posée par: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Répondue par: Technical Support 13
Date de publication: 2017-01-13
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_fr_FR, sid_376471, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 101ms
  • QUESTIONS, PRODUCT
Rated 5 de 5 de par 功能不错,敏感度很高,很好跑半年前试用了一周,感觉效果很棒,再次购买发现没让人失望,效果真的很不错,以后还会再买他家的抗体,都可以试试。
Date de publication: 2023-10-11
Rated 4 de 5 de par 经理BACKGROUND The Fox family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXM1, also known as FKHL16, MPP2 or Trident, is primarily expressed in proliferating cells. The gene encoding human FOXM1 maps to chromosome 12p13.33. The transcription element that restricts FOXM1 expression to prolif- erating cells is located 300 bp upstream of the start codon. FOXM1 is most abundant in thymus, testis, small intestine and colon. Alternative splicing generates FOXM1A and FOXM1B isoforms that contain PEST regions involved in rapid protein degradation. A decrease in FOXM1 expression is associated with age-related defects in cellular proliferation. Conversely, an increase in FOXM1B expression in the livers of older transgenic mice restore hepatocyte DNA replication rates to the higher rate present in young livers. FOXM1B activates the transcription of cyclin B1, cyclin D1 and Cdc25B
Date de publication: 2021-06-23
Rated 5 de 5 de par the greatest Ab i ever had!it works very well in both KD and OE systems i set up. believe me, commercial ABs of FOXM1 from other companies dont work at all. this showed clear band, at 1:250 dilution with senescent dermal fibroblast cell samples.
Date de publication: 2020-07-22
Rated 1 de 5 de par Not specific for FOXM1 - Do not use this antibody!As noticed by other users before, the FOXM1 G-5 antibody detects a protein with the same molecular weight as FOXM1 in Western Blots, but
Date de publication: 2018-07-18
Rated 4 de 5 de par Great antibodiesI got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date de publication: 2018-07-14
Rated 1 de 5 de par Not sure if observed band is FOXM1Like the previous comments below, our group has also confirmed that this antibody may not be recognizing FOXM1. We observe a strong clear band at around 125-130 kDa with this antibody in our cell lines. However, upon using both siRNA and shRNA approaches with 3 different species, we could not observe a clear knockdown of FOXM1 with this antibody. In contrast, FOXM1 (A-11) sc-271746 showed knockdown of FOXM1 protein. Additionally, while this antibody can recognize overexpressed FOXM1, the exogenous FOXM1 appears as a second at around 110 kDa. The FOXM1 (A-11) antibody only recognizes a single band with much greater intensity. The FOXM1 (A-11) antibody also gave consistent results with CS #D12D5 and an Abcam antibody.
Date de publication: 2018-05-11
Rated 3 de 5 de par Gives good band, but uncertain about specificityThe mouse G-5 antibody appears to detect primarily nuclear FOXM1; whereas the SC-500 rabbit antibody detects mainly cytoplasmic (and to some extent nuclear). The expression pattern in western blots, ICC, and IHC have confirmed this (images attached). Possibly due to the G-5 antibody unable to detect FOXM1 in cytoplasm if dimerized? It seems to detect FOXM1 differently in regards to localization; however, the MW appears to be correct. I still haven't quite figured this one out. In any case, I used the antibody at a 1:1000 dilution and secondary at 1:2500. I've noticed most SC antibodies work well at 1:250/500 primary and secondary at 1:2500. It has worked well with overexpression (human FOXM1b isoform) as well as siRNA knockdown in rat cells.
Date de publication: 2018-03-26
Rated 1 de 5 de par Not sure the band we detect is FOXM1We have observed similar results than the one showed by elenaenzo with two different siRNA against FOXM1 in HeLa cells (an antibody against FOXM1 from another firm was used as a positive control on the same lysates).
Date de publication: 2018-03-16
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_28
  • loc_fr_FR, sid_376471, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT
Anticorps FOXM1 (G-5) est évalué 4.0 de 5 de 28.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_28
  • loc_fr_FR, sid_376471, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 104ms
  • REVIEWS, PRODUCT