FN3K Double Nickase Plasmid (h): sc-412985-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FN3K Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FN3K Double Nickase Plasmid (h) and FN3K Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FN3K. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FN3K Antibody (E-9): sc-271503
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FN3K Double Nickase Plasmid (h) | sc-412985-NIC | 20 µg | $410.00 | |||
FN3K Double Nickase Plasmid (h2) | sc-412985-NIC-2 | 20 µg | $410.00 |
Human FN3K encodes fructosamine-3-kinase, a cytosolic deglycation enzyme that phosphorylates Amadori products on glycated proteins to promote their destabilization and removal, helping maintain proteostasis under carbonyl and glycation stress. FN3K activity intersects with glucose-derived reactive metabolite handling, oxidative stress responses, and protein quality control pathways that influence cellular aging and redox balance. Altered FN3K expression or activity has been associated with variability in protein glycation burden and has been explored in the context of metabolic dysregulation and diabetes-related molecular phenotypes. In biomedical research, FN3K is studied for its impact on glycated protein turnover, signaling perturbations caused by non-enzymatic glycation, and downstream effects on cell viability and stress adaptation.
FN3K Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FN3K locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FN3K. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FN3K function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FN3K-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.