FLRT3 CRISPR/Cas9 KO Plasmid (h): sc-406632
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FLRT3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FLRT3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FLRT3 Antibody (A-3): sc-514482
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FLRT3 CRISPR/Cas9 KO Plasmid (h) | sc-406632 | 20 µg | $397.00 |
Overview
FLRT3 (fibronectin leucine rich transmembrane protein 3) encodes a single-pass membrane protein that functions as a cell-adhesion and guidance cue during development, coordinating cell–cell interactions and neurite outgrowth. FLRT3 participates in signaling networks involving FGF receptor pathways and extracellular guidance ligands such as UNC5 receptors, influencing adhesion, repulsion, and tissue patterning. Through these interactions, FLRT3 contributes to regulation of cell migration, synapse formation, and morphogenesis in multiple tissue contexts. Altered FLRT3 expression and pathway connectivity have been reported in studies of neurodevelopmental processes and cancer biology, supporting its use as a mechanistic node in adhesion and migration research.
FLRT3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FLRT3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FLRT3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FLRT3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FLRT3 protein expression.
This CRISPR knockout system enables efficient generation of FLRT3-deficient cell models for investigation of FLRT3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FLRT3 exon(s) critical for FLRT3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FLRT3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FLRT3 CRISPR/Cas9 KO Plasmid (h) and FLRT3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FLRT3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FLRT3 HDR Plasmid (h) and FLRT3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FLRT3 homology arms to support homology-directed repair at defined FLRT3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.