Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Fibrinogen α CRISPR/Cas9 KO Plasmid (h): sc-400793
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fibrinogen α CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Fibrinogen α genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fibrinogen α Antibody (C-7): sc-398806
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fibrinogen α CRISPR/Cas9 KO Plasmid (h) | sc-400793 | 20 µg | $397.00 |
Overview
FGA encodes the fibrinogen α chain, a liver-derived secreted glycoprotein that assembles with the β and γ chains to form fibrinogen, the soluble precursor of fibrin. Upon vascular injury, thrombin-mediated cleavage of fibrinogen initiates polymerization and crosslinking events that stabilize clots and support platelet adhesion within the coagulation cascade. Fibrin(ogen) also participates in extracellular matrix remodeling and inflammatory signaling through interactions with integrins and other receptors, linking hemostasis to wound repair. Altered FGA function or expression is associated with quantitative or qualitative fibrinogen abnormalities that affect clot formation and can modify thrombotic or bleeding phenotypes, making it relevant for studies of coagulation biology and vascular inflammation.
Fibrinogen α CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FGA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FGA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FGA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Fibrinogen α protein expression.
This CRISPR knockout system enables efficient generation of FGA-deficient cell models for investigation of Fibrinogen α signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FGA exon(s) critical for Fibrinogen α function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FGA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Fibrinogen α CRISPR/Cas9 KO Plasmid (h) and Fibrinogen α CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FGA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Fibrinogen α HDR Plasmid (h) and Fibrinogen α HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FGA homology arms to support homology-directed repair at defined FGA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.