FEZF2 CRISPR/Cas9 KO Plasmid (h): sc-406571
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FEZF2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FEZF2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FEZF2 CRISPR/Cas9 KO Plasmid (h) | sc-406571 | 20 µg | $397.00 |
Overview
FEZF2 (FEZ family zinc finger 2) encodes a transcription factor with C2H2-type zinc finger domains that functions as a key regulator of forebrain development and neuronal subtype specification. In human neurodevelopment, FEZF2 influences corticogenesis by directing corticothalamic projection neuron fate and coordinating transcriptional programs linked to axon guidance, neuronal migration, and regional patterning. Through its role in lineage-defining gene networks, FEZF2 contributes to the establishment of cortical circuitry and synaptic connectivity. Dysregulation or genetic variation in FEZF2-associated regulatory pathways has been investigated in the context of neurodevelopmental and neuropsychiatric phenotypes, making it relevant for mechanistic studies of brain development and neuronal identity.
FEZF2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FEZF2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FEZF2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FEZF2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FEZF2 protein expression.
This CRISPR knockout system enables efficient generation of FEZF2-deficient cell models for investigation of FEZF2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FEZF2 exon(s) critical for FEZF2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FEZF2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FEZF2 CRISPR/Cas9 KO Plasmid (h) and FEZF2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FEZF2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FEZF2 HDR Plasmid (h) and FEZF2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FEZF2 homology arms to support homology-directed repair at defined FEZF2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.