Date published: 2026-7-11

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FADD Antibody (H-10): sc-271520

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Datasheets
  • FADD Antibody (H-10) is a mouse monoclonal IgG2a κ, cited in 7 publications, provided at 200 µg/ml
  • raised against amino acids 28-209 mapping at the C-terminus of FADD of human origin
  • recommended for detection of FADD of human origin by WB, IP, IF and ELISA
  • See FADD (G-4): sc-271748 for FADD antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for FADD Antibody (H-10). This work is in progress.
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FADD Antibody (H-10) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect the Fas-Associated Death Domain (FADD), also known as GIG3, or MORT1. FADD monoclonal antibody (H-10) targets an epitope spanning amino acids 28-209 at the C-terminus of human FADD, ensuring high specificity and reliable binding. FADD is predominantly localized in the cytoplasm, where FADD plays a crucial role in apoptotic signaling by linking death receptors such as FAS and TNFR1 to downstream caspases, thereby facilitating programmed cell death. This cytoplasmic localization is essential for maintaining cellular homeostasis, regulating immune responses, and preventing uncontrolled cell proliferation, which is vital in cancer and autoimmune disease research. Anti-FADD antibody (H-10) is validated for the detection of human FADD in various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). By enabling precise investigation of FADD′s cellular functions and interactions, FADD (H-10) antibody serves as an indispensable tool for researchers studying the mechanisms of apoptosis and its implications in health and disease. Anti-FADD antibody (H-10) high affinity and specificity make FADD ideal for elucidating the complex pathways involved in cell death and survival. Whether exploring fundamental biological processes or developing therapeutic strategies, FADD monoclonal antibody (H-10) provides reliable performance and consistent results.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

FADD Antibody (H-10)

sc-271520
200 µg/ml
$322.00

What is the recommended starting dilution for Western blot for sc-271520: FADD (H-10) monoclonal antibody?

Asked by: Trav11
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Satisfactory Western Blot data of FADD expressionSatisfactory Western Blot data of FADD expression in SW480, THP-1 and A-431 whole cell lysates. -SCBT QC
Date published: 2015-02-10
Rated 5 out of 5 by from Produced positive Western Blot data of FADDProduced positive Western Blot data of FADD expression in THP-1 whole cell lysate. -SCBT QC
Date published: 2014-01-26
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FADD Antibody (H-10) is rated 5.0 out of 5 by 2.
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