F4/80 Antibody (BM8): sc-52664
- F4/80 Antibody (BM8) is a rat monoclonal IgG2a, cited in 107 publications, provided at 100 µg/ml
- raised against cultured bone marrow-derived macrophages of mouse origin
- recommended for detection of EMR1 of human origin, F4/80 of mouse origin and the corresponding rat homolog by WB, IF, IHC(P) and FCM
- See F4/80 (C-7): sc-377009 for F4/80 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for F4/80 Antibody (BM8). This work is in progress.
F4/80 Antibody (BM8) is a rat monoclonal IgG2a antibody that detects the EMR1 protein of human origin, F4/80 protein of mouse origin, and the corresponding rat homolog by western blotting (WB), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). F4/80 (BM8) antibody is available in a non-conjugated form, making F4/80 (BM8) monoclonal antibody versatile for various experimental setups. F4/80 protein, also known as EGF-like molecule containing mucin-like hormone receptor 1, is a member of the epidermal growth factor (EGF)-TM7 family, which includes several class B G-protein coupled receptors predominantly expressed on immune cells. Notably, F4/80 is exclusively found on the surface of macrophages, serving as a critical marker for mature macrophage populations in tissues such as Kupffer cells in the liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin. F4/80 protein undergoes significant post-translational modifications, including extensive N-linked glycosylation and some O-linked glycosylation, which are essential for stability, localization, and function. While the precise role of F4/80/EMR1 remains to be fully elucidated, F4/80 plays a vital part in macrophage adhesion, migration, and potentially in G-protein coupled signaling pathways, highlighting F4/80′s importance in immune responses and tissue homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
F4/80 Antibody (BM8) References:
- F4/80 monoclonal antibody. | Hume, D. 1992. J Leukoc Biol. 51: 517-8. PMID: 1376355
- A function for the macrophage F4/80 molecule in tolerance induction. | van den Berg, TK. and Kraal, G. 2005. Trends Immunol. 26: 506-9. PMID: 16087400
- F4/80: the macrophage-specific adhesion-GPCR and its role in immunoregulation. | Lin, HH., et al. 2010. Adv Exp Med Biol. 706: 149-56. PMID: 21618834
- F4/80 and the related adhesion-GPCRs. | Gordon, S., et al. 2011. Eur J Immunol. 41: 2472-6. PMID: 21952799
- ¹¹¹In-anti-F4/80-A3-1 antibody: a novel tracer to image macrophages. | Terry, SY., et al. 2015. Eur J Nucl Med Mol Imaging. 42: 1430-8. PMID: 26012900
- F4/80+ Kupffer Cell-Derived Oncostatin M Sustains the Progression Phase of Liver Regeneration through Inhibition of TGF-β2 Pathway. | Lu, Q., et al. 2021. Molecules. 26: PMID: 33924385
- Anti-F4/80 treatment attenuates Th2 cell responses: Implications for the role of lung interstitial macrophages in the asthmatic mice. | Deng, N., et al. 2021. Int Immunopharmacol. 99: 108009. PMID: 34315114
- Periosteal CD68+ F4/80+ Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-β1. | Deng, R., et al. 2022. Adv Sci (Weinh). 9: e2103343. PMID: 34854257
- F4/80hi Resident Macrophages Contribute to Cisplatin-Induced Renal Fibrosis. | Sears, SM., et al. 2022. Kidney360. 3: 818-833. PMID: 36128491
- F4/80+Ly6Chigh Macrophages Lead to Cell Plasticity and Cancer Initiation in Colitis. | Shin, AE., et al. 2023. Gastroenterology. 164: 593-609.e13. PMID: 36634827
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
F4/80 Antibody (BM8) | sc-52664 | 100 µg/ml | $322.00 |