Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EXOSC4 CRISPR/Cas9 KO Plasmid (h): sc-405751
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EXOSC4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EXOSC4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EXOSC4 Antibody (G-9): sc-166772
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EXOSC4 CRISPR/Cas9 KO Plasmid (h) | sc-405751 | 20 µg | $397.00 |
Overview
EXOSC4 encodes a core component of the RNA exosome complex, a multi-subunit 3′–5′ exoribonuclease that governs RNA surveillance and turnover in the nucleus and cytoplasm. By supporting processing and degradation of diverse RNA species, including rRNA, sn/snoRNA, and aberrant or unstable transcripts, EXOSC4 contributes to RNA quality control, ribosome biogenesis, and maintenance of transcriptome homeostasis. Exosome function intersects with co-transcriptional RNA processing, nuclear export, and RNA decay pathways that shape gene expression programs. Dysregulation of exosome-associated factors has been linked to altered RNA metabolism and cellular stress responses, making EXOSC4 a relevant node for mechanistic studies of RNA processing defects in disease-relevant contexts.
EXOSC4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EXOSC4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EXOSC4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EXOSC4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EXOSC4 protein expression.
This CRISPR knockout system enables efficient generation of EXOSC4-deficient cell models for investigation of EXOSC4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EXOSC4 exon(s) critical for EXOSC4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EXOSC4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EXOSC4 CRISPR/Cas9 KO Plasmid (h) and EXOSC4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EXOSC4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EXOSC4 HDR Plasmid (h) and EXOSC4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EXOSC4 homology arms to support homology-directed repair at defined EXOSC4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.