Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ETEA Double Nickase Plasmid (h): sc-403730-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ETEA Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ETEA Double Nickase Plasmid (h) and ETEA Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FAF2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ETEA Antibody (F-7): sc-374098
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ETEA Double Nickase Plasmid (h) | sc-403730-NIC | 20 µg | $410.00 | |||
ETEA Double Nickase Plasmid (h2) | sc-403730-NIC-2 | 20 µg | $410.00 |
FAF2 encodes the endoplasmic reticulum–resident protein ETEA (also known as UBXD8), a UBX-domain adaptor that links ubiquitinated substrates to the p97/VCP ATPase to promote ER-associated degradation (ERAD). Through coordination with ER membrane ubiquitin ligases and lipid droplet–associated machinery, ETEA influences proteostasis, unfolded protein stress responses, and lipid metabolism, including turnover of key regulators of fatty acid and sterol homeostasis. Altered FAF2/ETEA activity has been connected to dysregulated ER stress signaling and metabolic remodeling observed in contexts such as cancer cell adaptation and hepatic lipid disorders, making it relevant for studying stress tolerance and membrane lipid regulation.
ETEA Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FAF2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FAF2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FAF2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FAF2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.