ERp57 Antibody (TO-2): sc-80648

ERp57 Antibody (TO-2) is a mouse monoclonal IgG1 antibody that detects ERp57 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). ERp57, also known as GRP57, GRP58, ERp60, and ERp61, plays a crucial role in the major histocompatibility complex (MHC) class I pathway, which is essential for the immune response against intracellular pathogens. ERp57 is primarily located in the endoplasmic reticulum, where ERp57 assists in the proper folding and assembly of MHC class I molecules, ensuring that MHC class I molecules can effectively present short pathogenic peptides to cytotoxic T lymphocytes. Proper functioning of ERp57 is vital, as any disruption in this process can lead to impaired immune responses and increased susceptibility to infections. The human ERp57 gene is located on chromosome 15q15 and encodes a 505 amino acid protein that contains two highly conserved Trp-Cys-Gly-His-Cys-Lys motifs, underscoring ERp57′s evolutionary importance. Additionally, ERp57 exhibits multifunctional properties, acting as a protease, protein disulfide isomerase, and potentially a phospholipase, which further highlights ERp57′s significance in cellular processes. With broad species reactivity and versatile applications, anti-ERp57 antibody (TO-2) is an invaluable tool for researchers studying immune responses and protein interactions in various biological contexts.

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ERp57 Antibody (TO-2) References:

1. The ERp57/GRp58/1,25D3-MARRS receptor: multiple functional roles in diverse cell systems.  |  Khanal, RC. and Nemere, I. 2007. Curr Med Chem. 14: 1087-93. PMID: 17456022
2. The co-translocation of ERp57 and calreticulin determines the immunogenicity of cell death.  |  Panaretakis, T., et al. 2008. Cell Death Differ. 15: 1499-509. PMID: 18464797
3. ERp57 modulates STAT3 signaling from the lumen of the endoplasmic reticulum.  |  Coe, H., et al. 2010. J Biol Chem. 285: 6725-38. PMID: 20022947
4. The disulfide isomerase ERp57 mediates platelet aggregation, hemostasis, and thrombosis.  |  Wu, Y., et al. 2012. Blood. 119: 1737-46. PMID: 22207737
5. ERp57‑small interfering RNA silencing can enhance the sensitivity of drug‑resistant human ovarian cancer cells to paclitaxel.  |  Li, S., et al. 2019. Int J Oncol. 54: 249-260. PMID: 30431082
6. Integrins and ERp57 Coordinate to Regulate Cell Surface Calreticulin in Immunogenic Cell Death.  |  Liu, CC., et al. 2019. Front Oncol. 9: 411. PMID: 31192123
7. Recognition Dynamics of Cancer Mutations on the ERp57-Tapasin Interface.  |  Padariya, M., et al. 2020. Cancers (Basel). 12: PMID: 32244998
8. Cancer Biology of the Endoplasmic Reticulum Lectin Chaperones Calreticulin, Calnexin and PDIA3/ERp57.  |  Lam, STT. and Lim, CJ. 2021. Prog Mol Subcell Biol. 59: 181-196. PMID: 34050867
9. Identification of dihydrotanshinone I as an ERp57 inhibitor with anti-breast cancer properties via the UPR pathway.  |  Shi, W., et al. 2021. Biochem Pharmacol. 190: 114637. PMID: 34062127
10. Structure of an MHC I-tapasin-ERp57 editing complex defines chaperone promiscuity.  |  Müller, IK., et al. 2022. Nat Commun. 13: 5383. PMID: 36104323

The preferred ERp57 antibody is ERp57 Antibody (MaP.ERp57): sc-23886.