Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
eIF4H CRISPR/Cas9 KO Plasmid (h): sc-411056
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF4H CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the eIF4H genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF4H Antibody (C-6): sc-515265
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF4H CRISPR/Cas9 KO Plasmid (h) | sc-411056 | 20 µg | $397.00 |
Overview
EIF4H encodes the human eIF4H translation initiation factor, an accessory protein that cooperates with eIF4A to stimulate ATP-dependent RNA helicase activity and promote scanning through structured 5′ UTRs during cap-dependent translation initiation. By tuning ribosome recruitment and mRNA-specific translational efficiency, eIF4H helps shape proteome output in response to growth cues and cellular stress, linking EIF4H function to regulation of proliferation, differentiation, and stress-adaptive programs. Dysregulation of translation initiation components, including eIF4H, has been associated with altered expression of oncogenic and survival pathways and is frequently investigated in contexts such as tumor biology, neurobiology, and virus–host translation control. EIF4H perturbation therefore provides a useful entry point for dissecting translational control mechanisms and their downstream signaling consequences.
eIF4H CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EIF4H gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EIF4H together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EIF4H open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish eIF4H protein expression.
This CRISPR knockout system enables efficient generation of EIF4H-deficient cell models for investigation of eIF4H signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EIF4H exon(s) critical for eIF4H function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EIF4H genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by eIF4H CRISPR/Cas9 KO Plasmid (h) and eIF4H CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EIF4H locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by eIF4H HDR Plasmid (h) and eIF4H HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EIF4H homology arms to support homology-directed repair at defined EIF4H target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.