Date published: 2025-9-9

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Anticorps eIF2α (G-12): sc-133227

4.8(5)
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Fiches techniques
  • L'Anticorps eIF2α (G-12) est un monoclonal de souris IgG2b κ, cité dans 23 publications, fourni en 200 µg/ml
  • dirigé contre les acides aminés 1-315 correspondant à la séquence totale de eIF2α d'origine human
  • recommandé pour la détection de eIF2α d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
  • Voir eIF2α (D-3): sc-133132 pour d'autres anticorps conjugués eIF2α, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
  • m-IgG2b BP-HRP est le réactif secondaire recommandé pour la détection de l'anticorps eIF2α (G-12) pour des applications de WB and IHC(P). Ce réactif est maintenant proposé en kit avec l'anticorps eIF2α (G-12) (voir Informations pour la commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2α, eIF2β and eIF2γ (eukaryotic translation initiation factor 2 α, β and γ, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2α, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2α controls the rate of tRNA translation. When eIF2α is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2α is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps eIF2α (G-12)

sc-133227
200 µg/ml
$316.00

eIF2α (G-12): m-IgG2b BP-HRP Kit

sc-548488
200 µg Ab; 10 µg BP
$354.00

I am using eIF2α (G-12): sc-133227 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Posée par: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 5 de 5 de par Great productI have just tried this antibody and it gave me highly specific bands at the size that is mentioned and expected for eIF2alpha. I tried it at a dilution of 1:100 in 5% NFDM prepared in TBST.
Date de publication: 2020-08-07
Rated 4 de 5 de par GreatA good antibody to detect human eIF2a in 293T cells
Date de publication: 2018-11-19
Rated 5 de 5 de par can be usedused in the wb, meth-induced apoptosis, the band is clear.
Date de publication: 2017-03-07
Rated 5 de 5 de par Strong cytoplasmic staining of glandularStrong cytoplasmic staining of glandular cells in IHC(P) with human rectum tissue -SCBT QC
Date de publication: 2015-04-10
Rated 5 de 5 de par Robust bands with 293TRobust bands with 293T, HeLa, K-562, NIH/3T3 and KNRK whole cell lysates in WB -SCBT QC
Date de publication: 2014-10-06
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Anticorps eIF2α (G-12) est évalué 4.8 de 5 de 5.
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